Caspase-3 Substrate (Fluorogenic), Ac-DEVD-AFC

SKU
C-1127
  • CAS:201608-14-2
  • Formula:C30H34N5O13F3
  • MW:729.62 Da
  • Appearance:White Powder
  • Purity:≥98%

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Product Name Qty
Caspase Substrate (AFC), Ac-DEVD-AFC
1 mg
$58.07
Caspase Substrate (AFC), Ac-DEVD-AFC
5 mg
$167.51

Fluorogenic substrate for caspase-3, with K=9.7M. C1127 issimilar to Ac-DEVD-AMC (# C-1133) but the AFC fluorophore has a greater Stokes shift upon cleavage. The peptide sequence is based on the PARP cleavage site Asp216 for caspase-3. Ex.:400nm, Em.: 505nm.

More Information
Alternate Name/Synonyms
Ac-DEVD-AFC; Caspase-3 Substrate (Fluorogenic); N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin
SKU
C-1127
CAS #
201608-14-2
Chemical Name
(4S)-4-[[(2S)-2-acetamido-3-carboxypropanoyl]amino]-5-[[(2S)-1-[[(2S)-3-carboxy-1-oxo-1-[[2-oxo-4-(trifluoromethyl)chromen-7-yl]amino]propan-2-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-5-oxopentanoic acid
Chemical Formula
C30H34N5O13F3
Molecular Weight
729.62 Da
Appearance
White Powder
Purity
≥98%
Solubility
Soluble in DMSO, dimethyl formamide or methanol; slightly soluble in water (0.4 mg/ml)
Melting Point
N/A
Preparation
N/A
Storage Temp
-20°C, keep cool and dry
THERAPEUTIC AREA
Not Available
USE
Ac-DEVD-AFC is a fluorogenic substrate for caspase-3 (CPP32) and related cysteine proteases, which emits a blue to green shift in fluorescence. It also acts as a substrate for granzyme B-processed caspase-6, caspase-7, caspase-8, and caspase-10. It is known that Ac-DEVD-AFC has a greater Stokes' shift than Ac-DEVD-AMC once it has been cleaved. Since caspase-3 is an effector caspase, it is involved in the cleavage of many substrates which promote apoptosis. This indicator excites light at 400 nm and emits light at 505 nm. In addition, Ac-DEVD-AFC is modeled after Asp216 on caspase-3, the PARP cleavage site. Ac-DEVD-AFC is an essential indicator used to monitor the cellular effects of caspase-3.
Merck Index
N/A
MDL NUMBER
MFCD01310970
CHEMACX
X1206324-4
INCHI
InChI=1S/C30H34F3N5O13/c1-12(2)25(38-26(47)17(6-7-21(40)41)36-28(49)18(10-22(42)43)34-13(3)39)29(50)37-19(11-23(44)45)27(48)35-14-4-5-15-16(30(31,32)33)9-24(46)51-20(15)8-14/h4-5,8-9,12,17-19,25H,6-7,10-11H2,1-3H3,(H,34,39)(H,35,48)(H,36,49)(H,37,50)(H,38,47)(H,40,41)(H,42,43)(H,44,45)/t17-,18-,19-,25-/m0/s1
SMILES
CC(C)[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)Nc1ccc2c(cc(=O)oc2c1)C(F)(F)F)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)C
Peptide sequence
AC-ASP-GLU-VAL-ASP-7-AFC
RTECS
Not Applicable
UN #'S
Not Applicable
PACKING GROUP
Not Applicable
HAZARD CLASS
Not Applicable
Handling
Corrosive hazard. Wear protective gloves/clothing and eye/face protection. Thermal decomposition can lead to release of toxic/corrosive gases and vapors.
Certificate of Analysis 1
Certificate of Analysis 2
GHS PICTOGRAMS
Handling Corrosive hazard. Wear protective gloves/clothing and eye/face protection. Thermal decomposition can lead to release of toxic/corrosive gases and vapors.
UN #'S Not Applicable
Packing Group Not Applicable
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