Highlights:

• Pre-activated medium for immobilization of ligands containing primary amines.
• N, N`-Carbonyl diimidazole (CDI) mediated activation of Separopore® 4B matrix to allow coupling of the primary amino ligands by a stable amide bond formation.
• No charge is introduced into the matrix after the coupling reaction.
• No ligand leakage after coupling.
• Superior stability: Activated matrix is much more resistant to hydrolysis.
• Ideal for immobilization of large protein ligands such as antibodies as well as peptides and nucleic acids.
• Coupling reaction can also be carried out in organic solvents.
• High coupling efficiency and easy to handle.
• CDI-activated Separopore® 4B is activated with CDI groups for covalent immobilization of proteins and other molecules that contain N-nucleophiles.

Technical specifications:

• Group to be coupled: -NH₂ groups
• Matrix: Separopore® 4B (agarose beads, 4%)
• Particle size range: 52 – 165 μm
• Molecular weight range: 6 x 10⁴ - 2 x 10⁷
• Coupling conditions: pH: 8.5 – 11, Temp: 4 – 25 °C, Time: 6 – 24 h
• pH stability: 11 (ligand dependent)