CHAPS 10% Solution, Sterile-Filtered

SKU
C-1020
  • CAS:75621-03-3
  • Formula:C32H58N2O7S
  • MW:614.9 Da
  • Appearance:10% solution in DI water
  • Purity:≥98%

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Product Name Qty
CHAPS 10% Solution, Sterile-Filtered
5 x 10 mL
$62.50
CHAPS 10% Solution, Sterile-Filtered
1 L
$249.99
CHAPS 10% Solution, Sterile-Filtered
100 mL
$75.00
CHAPS 10% Solution, Sterile-Filtered
50 mL
$49.99
CHAPS 10% Solution, Sterile-Filtered
500 mL
$199.99

 

Description

CHAPS Zwitterionic, is a non-denaturing surfactant derived from cholic acid. The primarily use of CHAPS is in solubilization and purification of membrane proteins and other macromolecules. It can be removed from gels or protein solutions by dialysis across a membrane. It combines the features of bile salts and N-alkyl sulfobetaine. CHAPS behaves as a weak charged cation when in salt, but when in water or buffer it reacts as a nonionic surfactant. This product is 10% sterile-filtered solution in water of the Zwitterionic detergent CHAPS.

 Applications

• For monitoring chemical reactions or protein-protein binding using UV/VIS spectroscopy 
• For ion exchange chromatography and isoelectric
• For membrane permeabilization of cells
• As a component in denaturing buffer in enzyme characterization

More Information
Alternate Name/Synonyms
CHAPS solution
SKU
C-1020
CAS #
75621-03-3
Chemical Name
3-[dimethyl-[3-[[(4R)-4-[(3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-3,7,12-trihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoyl]amino]propyl]azaniumyl]propane-1-sulfonate
Chemical Formula
C32H58N2O7S
Molecular Weight
614.9 Da
Appearance
10% solution in DI water
Purity
≥98%
Solubility
10% sterile-filtered solution in water of the Zwitterionic detergent CHAPS
Storage Temp
Room temperature
THERAPEUTIC AREA
Not Applicable
USE
CHAPS 10% Solution is a non-denaturing, zwitterionic detergent which is derived from cholic acid. It is primarily used for membrane permeabilization of cells and for protein solubilization in isoelectric focusing. CHAPS 10% Solution is used as a denaturing buffer component in enzyme characterization and 2D- sample buffer component in 2D electrophoresis. CHAPS 10% Solution is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12.
Merck Index
13.2052.2001
MDL NUMBER
MFCD00012116
CHEMACX
X1016609-1
INCHI
InChI=1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
SMILES
C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS(=O)(=O)[O-])[C@H]1CC[C@@H]2[C@@]1([C@H](C[C@H]3[C@H]2[C@@H](C[C@H]4[C@@]3(CC[C@H](C4)O)C)O)O)C
Handling
Ensure adequate ventilation, especially in confined areas. Use personal protective equipment as required. Use with local exhaust ventilation. Do not breathe dust/fume/gas/mist/vapors/spray. Handle in accordance with good industrial hygiene and safety practice. Corrosive hazard. Wear protective gloves/clothing and eye/face protection.
GHS PICTOGRAMS
Handling Ensure adequate ventilation, especially in confined areas. Use personal protective equipment as required. Use with local exhaust ventilation. Do not breathe dust/fume/gas/mist/vapors/spray. Handle in accordance with good industrial hygiene and safety practice. Corrosive hazard. Wear protective gloves/clothing and eye/face protection.
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