Cu2+-Chelated (Agarose) 4B-CL

  • Appearance:Liquid Suspension
Product Name Qty
Cu2+-Chelated (Agarose) 4B-CL
10 ml


Cu2+-chelated 4B-CL is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to Cu2+ metal ions. It is suitable for both laboratory scale purification and large-scale use.


  • Affinity matrix for proteins.
  • Imminodiacetate immobilized matrix charged with Cu2+ metal ions.
  • Ideal for purification of recombinant proteins containing (His)6-fusion tag.
  • It provides effective binding and selectivity for a wide range of proteins.
  • High salt concentrations can be used and it withstands effective and rigorous Cleaning-In-Procedure (CIP).


Technical specifications:

  • Ligand: Copper
  • Matrix: 4B-CL (crosslinked agarose beads, 4%)
  • Particle size range: 52 – 165 µm
  • Matrix activation: Epoxy
  • Spacer arm: 14 atoms
  • Ligand density: > 20 µmol Cu2+ / ml drained gel
  • Binding capacity: ~ 5 mg histidine-tagged protein / ml drained gel
  • pH stability: 3 – 12
  • Chemical Stability: 40 °C for 7 days in 0.01M HCl, 1M NaOH, and acetate buffer pH 4, 20% ethanol
  • Autoclavable at 121 °C in 20% ethanol 
  • Antimicrobial agent: 20% ethanol
  • Supplied as suspension in 20% ethanol
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