• CAS:66-81-9
  • Formula:C15H23NO4
  • MW:281.35 Da
  • Appearance:White or cream color powder
  • Purity:≥95.0%
Product Name Qty
1 g
5 g
10 g
25 g
50 g



Cycloheximide is an antibiotic produced by Streptomyces sp. that activates against yeasts and fungi. It inhibits protein synthesis thru DNA translation arrest in eukaryotes. Cycloheximide also inhibits chain initiation and chain elongation by acting on 60S subunits of eukaryote ribosome.


• Used as an inhibitor to study cell-free protein biosynthesis in eukaryotes
• Used to block ribosome-dependent in vivo polypeptide synthesis
• Used in genome editing utilizing the CRISPR/Cas9 system
• Used to clear macrophages in atherosclerotic plaques
• Used to activate cumulus-free equine oocytes, and to have neuroprotective properties

Cycloheximide is in stock. Call for bulk quotations, typical lead for subdivision, packing and shipping larger quantities is 7-14 days. Research size order 100mg 250mg lead is shorter.


More Information
Alternate Name/Synonyms
Actidione; Naramycin A; Kaken; Hizarocin; Zykloheximid; Neocycloheximide; Acti-Aid; Actispray
Chemical Name
Chemical Formula
Molecular Weight
281.35 Da
White or cream color powder
Soluble in Chloroform, Ethanol and Methanol
Store solutions at -20°C
Storage Temp
+4°C, desiccated
Infectious diseases
Cycloheximide is a highly effective antibiotic with activity against mold, yeast, and phytopathogenic fungi, with lower activity against bacteria. It has been reported to inhibit the synthesis of both proteins and macromolecules, as well as affect apoptosis in eukaryotes. Inhibition of protein synthesis is believed to be mediated through DNA translation arrest, as demonstrated in rat thymocytes. Although cycloheximide has been reported to induce apoptosis in various cells, it has also been shown to inhibit or delay induced apoptosis. These observations suggest that cycloheximide's effect on apoptosis may not be solely through protein translation arrest and that other mechanisms may be involved. Cycloheximide has also been reported to inhibit FKBP12 (peptidylprolylisomerase hFKBP12, PPIase hFKBP12) via competitive inhibition.
Peptide sequence
Not Applicable
UN #'S
UN 2811
Not Applicable
Avoid contact with skin, eyes or clothing. Wash contaminated clothing before reuse. Do not eat, drink or smoke when using this product. Use personal protective equipment as required. Do not breathe dust/fume/gas/mist/vapors/spray. Use with local exhaust ventilation. Corrosive hazard. Wear protective gloves/clothing and eye/face protection.
Certificate of Analysis 1
Certificate of Analysis 2
Certificate of Analysis 3
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HandlingAvoid contact with skin, eyes or clothing. Wash contaminated clothing before reuse. Do not eat, drink or smoke when using this product. Use personal protective equipment as required. Do not breathe dust/fume/gas/mist/vapors/spray. Use with local exhaust ventilation. Corrosive hazard. Wear protective gloves/clothing and eye/face protection.
UN #'SUN 2811
Packing GroupI
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Utilizing a Microbial Natural Product to Investigate Cellular Circuitry Governing Fungal Drug Resistance and Morphogenesis
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Mycological Characteristics of Ophiostoma quercus, a Sap-staining Fungus Isolated from Japanese Black Pine in Korea
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The potential roles of c-Jun N-terminal kinase (JNK) during the maturation and aging of oocytes produced by a marine protostome worm
AXL degradation in combination with EGFR-TKI can delay and overcome acquired resistance in human non-small cell lung cancer cells
Differential toxicity of ataxin-3 isoforms in Drosophila models of Spinocerebellar Ataxia Type 3
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Glucagon-Dependent Suppression of mTORC1 is Associated with Upregulation of Hepatic FGF21 mRNA Translation
Degron capability of the hydrophobic C-terminus of the polyglutamine disease protein, ataxin-3
Post-translational modifications of serine protease TMPRSS13 regulate zymogen activation, proteolytic activity, and cell surface localization
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