Cytochrome c Releasing Apoptosis Assay Kit

SKU
C-1367

2

1

0

Product Name Qty
Cytochrome c Releasing Apoptosis Assay Kit
100 Tests
$653.29

Description:

Cytochrome c plays an important role in apoptosis. The protein is located in the space between the inner and outer mitochondrial membranes. An apoptotic stimulus triggers the release of cytochrome c from the mitochondria into cytosol where it binds to Apaf-1. The cytochrome c/Apaf-1 complex activates caspase-9, which then activates caspase-3 and other downstream caspases. 

 

Application:

This assay kit is used to detect cytochrome c translocation from mitochrondria into cytosol during apoptosis. With an easy-to-use procedure, the kit includes reagents for separating a mitochondria-enriched fraction from cytosol. 

Cytochrome c released from mitochondria into cytosol can be determined by Western blotting using the cytochrome c antibody included in the kit. The procedure is simple and straightforward, and does not require ultracentrifugation.

 

Components:

• Mitochondria extraction buffer

• 5x cytosol extraction buffer

• DTT (1M)

• 500x protease inhibitor cocktail

• Anti-cytochrome c mouse mAb (0.2 mg/ml)

 

More Information
SKU
C-1367
Stability
6 months
Storage Temp
-20°C
Handling
Store in tightly sealed vial
GHS PICTOGRAMS
Handling Store in tightly sealed vial
We found other products you might like!
To Top