Description:

Cytochrome c plays an important role in apoptosis. The protein is located in the space between the inner and outer mitochondrial membranes. An apoptotic stimulus triggers the release of cytochrome c from the mitochondria into cytosol where it binds to Apaf-1. The cytochrome c/Apaf-1 complex activates caspase-9, which then activates caspase-3 and other downstream caspases. 

Application:

This assay kit is used to detect cytochrome c translocation from mitochrondria into cytosol during apoptosis. With an easy-to-use procedure, the kit includes reagents for separating a mitochondria-enriched fraction from cytosol. 

Cytochrome c released from mitochondria into cytosol can be determined by Western blotting using the cytochrome c antibody included in the kit. The procedure is simple and straightforward, and does not require ultracentrifugation.

Components:

• Mitochondria extraction buffer

• 5x cytosol extraction buffer

• DTT (1M)

• 500x protease inhibitor cocktail

• Anti-cytochrome c mouse mAb (0.2 mg/ml)