3,3'-Diaminobenzidine (DAB)

SKU
D-2561
  • CAS:91-95-2
  • Formula:C12H14N4
  • MW:214.27 Da
  • Appearance:Powder
  • Purity:≥98%

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3,3'-Diaminobenzidine [DAB]
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Description

3,3'-Diaminobenzidine (DAB) is an organic compound that is both chemically and thermodynamically stable. As its water-soluble tetrahydrochloride, DAB has been used in immunohistochemical staining of nucleic acids and proteins. Diaminobenzidine is oxidized by hydrogen peroxide in the presence of hemoglobin to give a dark-brown color. This is used to detect fingerprints in blood. 

A nucleic acid stain as well as a peroxidase substrate, 3,3’-Diaminoabenzidine (DAB) is a reagent for selenium spectrophotometric determination that can also be utilized for staining proteins.

Used for spectrophotometric determination of selenium.

More Information
Alternate Name/Synonyms
3,3′,4,4′-Biphenyltetramine; 3,3′,4,4′-Tetraaminobiphenyl; DAB
SKU
D-2561
CAS #
91-95-2
Chemical Name
4-(3,4-diaminophenyl)benzene-1,2-diamine
Chemical Formula
C12H14N4
Molecular Weight
214.27 Da
Appearance
Powder
Purity
≥98%
Solubility
Soluble in water (partly)
Melting Point
175-177 °C(lit.)
Storage Temp
4°C
THERAPEUTIC AREA
Not Applicable
USE
3,3'-Diaminobenzidine is a substrate for peroxidase and a reagent for spectrophotometric determination of selenium. It is used for the immunohistochemical staining of nucleic acids and proteins. It is also used as a chromogen for the visualization in immunohistochemistry of chondrosarcoma sections, mammary carcinomas and brain tissue sections.
Handling
Avoid contact with skin, eyes or clothing. Use personal protective equipment as required. Wash contaminated clothing before reuse. Do not breathe dust/fume/gas/mist/vapors/spray. Do not eat, drink or smoke when using this product. Handle in accordance with good industrial hygiene and safety practice. Corrosive hazard. Wear protective gloves/clothing and eye/face protection.
GHS PICTOGRAMS
Handling Avoid contact with skin, eyes or clothing. Use personal protective equipment as required. Wash contaminated clothing before reuse. Do not breathe dust/fume/gas/mist/vapors/spray. Do not eat, drink or smoke when using this product. Handle in accordance with good industrial hygiene and safety practice. Corrosive hazard. Wear protective gloves/clothing and eye/face protection.
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