Diaphorase (NADH)

SKU
N-1181
  • CAS:9032-20-6
  • MW:50 kDa (SDS-PAGE), 160 kDa (gel filtration)
  • Appearance:Yellowish amorphous powder, lyophilized

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Starting at $366.28

Product Name Qty
Diaphorase NADH
1 KU
$366.28

Description:

Diaphorase are dehydrogenase enzymes which catalyze the dehydrogenation of the reduced forms of the co-enzyme NADH and NADPH. NADH and NADPH originally come from purified extracts of Clostridium kluyveri cells, where diaphorase activity is shown. Other diaphorase activities come from various bacteria, plants and mammalian organs. This enzyme was isolated from Bacillus megaterium.

Applications:

• Used in the reoxidation of NADP to NADPH
• Used to construct a biosensor for acetaldehyde by immobilization
• Used in a study to assess the protein-protein interactions in assembly of lipoic acid on the 2-oxoacid dehydrogenases of aerobic metabolism

Specifications

• EC 1.6.5.2
• More than 70 U/mg solid
• Isoelectric point: pH 4.2
• Michaelis constant: NADH 5.5x10^-4 M
• Optimum pH: 8.0-9.0
• pH stability: 6.0-9.0 (37 C, 60 min)
• Optimum temperature: 40-45 C
• Thermal Stability: stable at 50 C and below (pH 8.0, 10 min)

 

More Information
Alternate Name/Synonyms
NAD(P)H Dehydrogenase (quinone); Diaphorase; DT-diaphorase; Azoreductase; Vitamin K reductase; Quinone reductase
SKU
N-1181
CAS #
9032-20-6
Chemical Name
Lipoamide dehydrogenase
Chemical Formula
N/A
Molecular Weight
50 kDa (SDS-PAGE), 160 kDa (gel filtration)
Appearance
Yellowish amorphous powder, lyophilized
Purity
N/A
Solubility
Soluble in water.
Melting Point
N/A
Preparation
N/A
Storage Temp
-20°C, desiccated
THERAPEUTIC AREA
Not Available
USE
Diaphorase (NADH) is useful for enzymatic determination of reduced NAD.
Merck Index
N/A
Handling
Follow good laboratory hygiene practices. Wear proper Personal Protective Equipment (PPE) and employ exposure controls as indicated in Section 8. Avoid physical contact. Minimize dust generation during use. Wash hands thoroughly after handling.
GHS PICTOGRAMS
Handling Follow good laboratory hygiene practices. Wear proper Personal Protective Equipment (PPE) and employ exposure controls as indicated in Section 8. Avoid physical contact. Minimize dust generation during use. Wash hands thoroughly after handling.
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