Dithiothreitol 1M Solution (DTT)

SKU
C-1030
  • CAS:3483-12-3
  • Formula:C4H10O2S2
  • MW:154.25 Da
  • Appearance:Clear colorless solution
  • Purity:≥99%

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Product Name Qty
DTT Solution (1 M)
250 mL
$325.00
DTT Solution (1 M)
10 mL
$35.00
DTT Solution (1 M)
50 mL
$80.00

 Description

DTT (Dithiothreitol) solution supplied as 1M in water, is a small-molecule redox reagent, known as Cleland's reagent, that forms a six-member ring with an internal disulfide bond. It has a redox potential of -0.33 V at pH 7. The reduction of typical disulfide bond proceeds by two sequential thiol-disulfide exchange reactions. DTT is effective in buffers to reduce protein disulfide bonds prior to SDS-PAGE. It can protect protein sulfhydryls and restore enzyme activity lost by oxidation.

Applications

• For reducing or "deprotecting" agent for thiolated DNA
• For reducing disulfide bonds of proteins 
• Used as an oxidizing agent
• Used as a component of medium for the demembranation and reactivation of spermatozoa
• Used to prevent intramolecular and intermolecular disulfide bonds from forming between cysteine residues of proteins

 

More Information
Alternate Name/Synonyms
DTT; Dithiothreitol; DL-Dithiothreitol solution; (±)-threo-1,4-Dimercapto-2,3-butanediol solution
SKU
C-1030
CAS #
3483-12-3
Chemical Name
(2S,3S)-1,4-bis(sulfanyl)butane-2,3-diol
Chemical Formula
C4H10O2S2
Molecular Weight
154.25 Da
Appearance
Clear colorless solution
Purity
≥99%
Solubility
Clear and colorless in water
USE
DTT is used as a reducing or "deprotecting" agent for thiolated DNA. The terminal sulfur atoms of thiolated DNA have a tendency to form dimers in solution, especially in the presence of oxygen. Dimerization greatly lowers the efficiency of subsequent coupling reactions such as DNA immobilization on gold in biosensors. Typically DTT is mixed with a DNA solution and allowed to react, and then is removed by filtration (for the solid catalyst) or by chromatography (for the liquid form). The DTT removal procedure is often called "desalting." Generally, DTT is used as a protecting agent that prevents oxidation of thiol groups.
Handling
Hygroscopic. Protect from moisture. Protect from light. The pharmacological and toxicological properties of this product have not been fully investigated. Use caution when handling. Do not use this compound if you are not fully trained or are unaware of the hazards involved.
GHS PICTOGRAMS
Handling Hygroscopic. Protect from moisture. Protect from light. The pharmacological and toxicological properties of this product have not been fully investigated. Use caution when handling. Do not use this compound if you are not fully trained or are unaware of the hazards involved.
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