EAH Separopore® (Agarose) 4B

  • Appearance:Liquid Suspension
Product Name Qty
EAH Separopore® (Agarose) 4B
5 ml

Applications: EAH-Separopore® 4B is used to couple molecules containing carboxyl groups to the matrix for affinity chromatography. It was prepared by covalent coupling of 1, 6-diamino hexane to Separopore® 4B via epoxy activation. The free terminal amino group allows for coupling of carboxyl-group containing ligands through amide bond formation by carbodimide-mediated coupling method. Due to the long hydrophilic spacer arm (10-atom), bio-World`s EAH-Separopore® 4B is ideal for immobilization of small molecules which are stable in low pH and contain free carboxyl groups.


Technical specifications:

  • Matrix active groups: -NH2 groups
  • Matrix: Separopore® 4B (agarose beads, 4%)
  • Particle size range: 52 – 165 μm
  • Matrix activation: Epoxy
  • Group to be coupled: -COOH groups
  • Chemical linkage of ligand: Stable amide linkage
  • Spacer arm: 6-amino caproic acid, 10 atoms
  • Ligand density: 7 – 11 μmol amino / ml medium
  • Coupling condition: pH 4.5 – 6, Temp: 4 – 25 °C
  • Storage: 20% ethanol containing 0.9% NaCl
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