ECH (Agarose) 4B

SKU
E-3335
  • Appearance:Liquid Suspension
Product Name Qty
ECH (Agarose) 4B
5 ml
$141.99

Highlights:

  • Affinity matrix for stable carbodiimide-based coupling of ligands containing amino groups.
  • Ligands are coupled in a single-step procedure in the presence of a coupling reagent (not provided) which is a water-soluble carbodiimide, such as N-ethyl-N`-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC).
  • The matrix provides a 10-atom spacer arm convenient for coupling of small ligands.
  • No blocking reaction is necessary following the coupling reaction.
  • Ideal for immobilization of small ligands stable in a low pH and containing primary amines.
  • Coupling reaction can be carried out in organic solvent.

 

Technical specifications:

  • Matrix active group: -COOH groups
  • Matrix: 4B (agarose beads, 4%)
  • Particle size range: 52 – 165 μm
  • Molecular weight range: 6 x 104 - 2 x 107
  • Group to be coupled: -NH2 groups
  • Chemical linkage of ligand: Stable amide linkage
  • Ligand density : 12 – 16 μmol carboxyl groups / ml drained matrix
  • Spacer arm: 6-amino caproic acid, 10 atoms
  • Coupling conditions pH 4.5 – 6, Temp: 4°C – 25 °C, Time: 1.5 – 24 h; organic solvents possible
  • Storage: 2 – 8 °C
More Information
SKU
E-3335
Appearance
Liquid Suspension
Storage Temp
4°C
Hazard Image
Yes
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