Epoxy activated (Agarose) 6B-CL

SKU
E-3343
  • Appearance:Liquid Suspension
Product Name Qty
Epoxy activated (Agarose) 6B-CL
1g
$167.63

Highlights:

  • Used in coupling of hydroxyl, amino or thiol groups to 6B-CL via a 12-carbon hydrophilic spacer arm.
  • Pre-activated medium for immobilization of ligands containing amino (primary or secondary), hydroxyl or thiol group.
  • Ideal for immobilization of alkali-resistant small ligands such as peptides, amino acids and carbohydrates.
  • Epoxy-activated 4B-CL is a pre-activated medium for coupling sugars and other carbohydrates via an ether linkage to a hydroxyl group.
  • Medium has a long hydrophilic 12 atom spacer arm which makes it particularly suitable for immobilization of small molecules.

 

Technical specifications:

  • Matrix active group: Epoxy
  • Matrix: 6B-CL (crosslinked agarose beads, 6%)
  • Particle size range: 52 – 165 μm
  • Group to be coupled: -NH2, -OH, or -SH
  • Molecular weight range: 6 x 104 – 2 x 107
  • Chemical linkage of ligand: Ether
  • Matrix spacer: 12 atoms (when ligands are coupled to free epoxy groups. Linkage is stable, uncharged ether)
  • Coupling conditions: pH 9 – 10 (amino ligands), pH 11 – 12 (hydroxyl ligands), pH 7.5 - 8.5 (thiol ligands); Temp: 4 – 37 °C; Time: 16 h to several days; organic solvents possible
  • pH stability: 2 – 11 (ligand dependent)
  • Storage: 2 – 8 °C
  • Supplied as lyophilized powder stabilized with lactose and dextran
  • 1g swells to 3 – 4 ml
More Information
SKU
E-3343
Appearance
Liquid Suspension
Storage Temp
4°C
Hazard Image
Yes
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