First Strand cDNA Synthesis Kit

SKU
F-2495

Starting at $249.99

Product Name Qty
First Strand cDNA Synthesis Kit
50 rxn
$249.99

Description

First Strand cDNA Synthesis Kit is an efficient & sensitive system for first strand cDNA synthesis from RNA templates. It includes all the necessary components to synthesize first-strand cDNA, except the template RNA (total RNA or mRNA).

First-Strand cDNA Synthesis Kit features two optimized mixes, RT Enzyme Mix and RT Reaction Mix. RT Enzyme Mix combines reverse transcriptase and RNase inhibitor, while RT Reaction Mix contains dNTPs, oligo(dT)18 primers, random hexamers, MgCl2 and an optimized buffer. The synthesized single-stranded cDNA is suitable for real-time quantitative PCR applications. The kit has been formulated to provide high yields of full-length cDNA product and to increase sensitivity in RT-qPCR. Starting material can range from 10 pg to 5 μg of total RNA.

 

Advantages & Features

• Convenient: the kit includes all the components needed
• Highly Sensitive: full length cDNA starting at 10 pg – 5 μg of total RNA, due a novel RNase H treatment
• Full-length first strand cDNA up to 10kb
• Optimized to increase sensitivity in RT-qPCR and RT-PCR assays
• Optimal reaction temperature: 50°C
• Avoids RNA template degradation

 

Applications

• First-strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full-length cDNA libraries
• RNA analysis

 

Specifications

• The performance of First-Strand cDNA Synthesis Kit is tested in an RT reaction using human total RNA. The sensitivity of the kit is verified by the detection of GAPDH transcript in 100 fg total RNA and the product generated is visualized on agarose gel.

 

*Developed and manufactured by Canvax Biotechnology, S.L under contractual agreement for AG Scientific, Inc.

More Information
SKU
F-2495
Storage Temp
Upon receipt of the kit, immediately store the components at -20°C in a freezer without a defrost cycle. It is recommended to reduce freeze-thaw cycles as much as possible.
USE
Efficient & sensitive complete system for first-strand cDNA synthesis from RNA templates
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