Glutathione (Agarose) 4B-CL, Super Flow
- Appearance:Supplied as 50% slurry in 20% ethanol
Reduced glutathione is covalently linked Agarose 4B for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. Glutathione Agarose Super Flow (SF) is used for protein purification by batch/column and scale-up procedures. Glutathione Agarose Super Flow (SF) has an exellent protein binding capacity. The good flow properties of the 92 μm (average size) highly crosslinked Agarose matrix makes an excellent choice for protein purification by column purification or batch process for scale-up.
This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Glutathione-Separopore is prepared by covalently coupling glutathione to epoxy-activated 4% crosslinked agarose beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity of 5 mg or more of Glutathione-S-transferase (GST) per ml of wet gel. Bound GST–fusion proteins are easily displaced from the resin by elution with buffers containing reduced glutathione. Mild elution conditions preserve protein antigenicity and function.
• Ligand: Reduced glutathione (GSH)
• Matrix: 4B-CL (highly crosslinked agarose beads, 4%)
• Particle size range: 52 – 165 µm
• Molecular weight range: 6 x 104 – 2 x 107
• Matrix activation: Epoxy
• Matrix spacer: 12 atoms
• Ligand density: 5 – 10 mg GST / ml drained gel
• Binding capacity: 5 – 10 mg recombinant GST-tagged protein / ml drained gel
• pH stability: 3 – 12
|Handling||Provide suitable ventilation. Wear any necessary personal protective equipment. Keep away from open flames, hot surfaces, and sources of ignition. Use spark-proof tools and explosion-proof equipment.|