Glutathione (Agarose) Microbeads

SKU
G-2623
  • Appearance:Supplied as suspension in 20% ethanol

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Product Name Qty
Glutathione (Agarose) Microbeads
10 mL
$209.10
Glutathione (Agarose) Microbeads
25 mL
$408.00

Description

Reduced glutathione is covalently linked Agarose 4B Microbeads for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Glutathione-Agarose is prepared by covalently coupling glutathione to epoxy-activated 4% crosslinked agarose beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity of 5 mg or more of Glutathione-S-transferase (GST) per ml of wet gel. Bound GST–fusion proteins are easily displaced from the resin by elution with buffers containing reduced glutathione. Mild elution conditions preserve protein antigenicity and function.

Specifications

• Matrix: Agarose microbeads, 4%
• Matrix Activation: Epoxy
• Particle Size Range: 25-50 μm
• Spacer Arm: 12 atoms
• Binding Capacity: 5-10 mg recombinant GST-tagged protein / mL drained gel
• Ligand: Reduced glutathione (GSH)
• Molecular Weight Range: 6 x 104 - 2 x 10

 

More Information
SKU
G-2623
Appearance
Supplied as suspension in 20% ethanol
Storage Temp
2-8°C
Handling
Provide suitable ventilation. Wear any necessary personal protective equipment. Keep away from open flames, hot surfaces, and sources of ignition. Use spark-proof tools and explosion-proof equipment.
GHS PICTOGRAMS
Handling Provide suitable ventilation. Wear any necessary personal protective equipment. Keep away from open flames, hot surfaces, and sources of ignition. Use spark-proof tools and explosion-proof equipment.
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