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IPTG

SKU
I-1312
  • CAS:367-93-1
  • Formula:C9H18O5S
  • MW:238.3 Da
  • Appearance:White crystalline powder
  • Purity:>99% (HPLC)

Starting at $17.51

Product Name Qty
IPTG
1 g
$17.51
IPTG
100 g
$288.78
IPTG
5 g
$21.39
IPTG
25 g
$93.05
IPTG
50 g
$149.74
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Description

IPTG known as Isopropyl-β-D-Thiogalactopyranoside functions as an inducer of galactosidase activity by binding to and inhibiting the repressor.

It is utilized for the induction of expression from the lac promoter and derivates. IPTG is also used to differentiate recombinants from non-recombinants in cloning strategies using vectors containing the Z or Z peptide gene.

 

Applications

• Used in cloning procedures that require induction of β-galactosidase activity
• Used in cellular systems to study gene activity
• Used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli

 

Additional Reading from AG Scientific

• IPTG: Frequently Asked Questions and Protocols

• IPTG Triggers Transcription of the Lac Operon

 

Not for human therapeutic use or for medicinal purposes. For research applications only.

More Information
Alternate Name/Synonyms
Isopropyl-beta-D-thiogalactoside; Isopropyl-beta-D-thiogalactopyranoside
SKU
I-1312
CAS #
367-93-1
Chemical Name
(2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-propan-2-ylsulfanyloxane-3,4,5-triol
Chemical Formula
C9H18O5S
Molecular Weight
238.3 Da
Appearance
White crystalline powder
Purity
>99% (HPLC)
Solubility
Soluble in water (5%), renders clear colorless solution
Melting Point
111 - 113ºC
Storage Temp
-20°C, desiccated
THERAPEUTIC AREA
Not Applicable
USE
IPTG, Dioxane-Free (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of the lac operon and induces expression of β-galactosidase in Escherichia coli. The compound is widely used with X-Gal (sc-280488) plates to screen for blue and white colonies. The lac operon system and IPTG have been used in multiple cloning experiments and cellular systems to study gene activity.
MDL NUMBER
MFCD00063273
CHEMACX
X1007022-5
INCHI
InChI=1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
SMILES
CC(C)S[C@H]1[C@@H]([C@H]([C@H]([C@H](O1)CO)O)O)O
Handling
Avoid contact with skin, eyes or clothing. Use personal protective equipment as required. Wash contaminated clothing before reuse. Do not breathe dust/fume/gas/mist/vapors/spray. Do not eat, drink or smoke when using this product. Use with local exhaust ventilation.
msds 1
I-1312, IPTG, SDS, diamond format, r01, 2020.pdf
Certificate of Analysis 1
I-1312, L1376.pdf
Certificate of Analysis 2
I-1312, K1220E.pdf
Certificate of Analysis 3
I-1312, J1109.pdf
GHS PICTOGRAMS
HandlingAvoid contact with skin, eyes or clothing. Use personal protective equipment as required. Wash contaminated clothing before reuse. Do not breathe dust/fume/gas/mist/vapors/spray. Do not eat, drink or smoke when using this product. Use with local exhaust ventilation.
CitationsEpithelial Na+ Channel: Reciprocal Control by COMMD10 and Nedd4-2
Identification of pre- and pro-haptens with a β-galactosidase-expressing E. coli culture system for skin sensitization
Recombinant HPV16 E7 assembled into particles induces an immune response and specific tumour protection administered without adjuvant in an animal model
Biosynthesis of methylated resveratrol analogs through the construction of an artificial biosynthetic pathway in E. coli
A β-galactosidase-expressing E. coli culture as an alternative test to identify skin sensitizers and non-sensitizers
USP5 Is Dispensable for Monoubiquitin Maintenance in Drosophila
Toxicity and aggregation of the polyglutamine disease protein, ataxin-3 is regulated by its binding to VCP/p97 in Drosophila melanogaster
Clonage, surexpression et purification de la RDH8 en fusion avec plusieurs étiquettes dans le but de mesurer son activité enzymatique
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