Lactacystin is a microbial metabolite of Streptomyces strains and acts as a cell permeable, specific, and irreversible inhibitor of 20S proteasome and lysosomal enzyme, Cathepsin A. It irreversibly binds to N-terminal threonine residue on active site of catalytic B subunit of 20S proteasome and therefore blocks proteolytic activity.
The metabolite blocks the cell cycle and induces neurite outgrowth in a murine neuroblastoma cell line, Neuro-2a. It inhibits cell cycle progression in both the G0/G1 and G2/M phases of the cell cycle. Treatment of Lactacystin in Neuro-2a cells displays bipolar morphology and becomes progressively more multipolar.
It has been reported that lactacystin induces apoptosis in human monoblastic U937, confirming DNA fragmentation.
• Proteasome inhibitor
• Study of neuritogenesis
Not for human therapeutic use or for medicinal purposes. For research applications only.
|Handling||Keep container tightly closed in a dry and well-ventilated place. Provide appropriate exhaust ventilation at places where dust is formed.|