Laemmli Buffer Reducing, 4X Solution

SKU
L-2411
  • CAS:56-81-5; 151-21-3; 60-24-2; 5704-04-1; 115-39-9; 7732-18-5
  • Appearance:Blue liquid

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Product Name Qty
Laemmli Buffer Reducing, 4X Solution
50 mL
$75.55

Description

Laemmli Buffer Reducing, 4X Solution is an SDS-containing buffer that can be used for preparation of protein samples for SDS-PAGE. The β-mercaptoethanol in the solution reduces intra- and inter-molecular disulfide bonds, allowing separation by size. The SDS detergent binds to the positive charges of the proteins, giving each protein the same negative charge. The SDS also denatures the proteins and their subunits, aiding in separation by size. The included bromophenol blue serves an indicator dye for easy migration tracking and allows for easy observation when loading gel wellls, providing minimal loss of protein samples. DTT is present in many buffer formulations to reduce disulphide bonds that can contribute to protein structures.

 

Applications

• SDS-PAGE protein sample preparation (denature proteins)

 

Specifications

• 4X Formulation: SDS: 8.0%, Glycerol: 40.0%, β-mercaptoethanol: 8.0%, Bromophenol Blue: 0.02%, Tris-HCl: 250 mM
• SDS binds proteins at a rate ~1.3 g SDS / g protein
• pH: 6.5-7.5

 

More Information
SKU
L-2411
CAS #
56-81-5; 151-21-3; 60-24-2; 5704-04-1; 115-39-9; 7732-18-5
Chemical Name
N/A
Chemical Formula
N/A
Molecular Weight
N/A
Appearance
Blue liquid
Purity
N/A
Solubility
Soluble in water
Melting Point
N/A
Preparation
N/A
Storage Temp
Room temperature
THERAPEUTIC AREA
Not Applicable
USE
Laemmli sample buffer is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The 2-mercaptoethanol reduces the intra and inter-molecular disulfide bonds.
Merck Index
N/A
RTECS
Not Applicable
UN #'S
Not Applicable
PACKING GROUP
Not Applicable
HAZARD CLASS
Not Applicable
Handling
Use in a chemical fume hood, with air supplied by an independent system. Avoid inhalation, contact with eyes, skin and clothing. Avoid the formation of dust and aerosols. Use in a wellventilated area. Keep away from sources of ignition. Avoid prolonged or repeated exposure.
GHS PICTOGRAMS
Handling Use in a chemical fume hood, with air supplied by an independent system. Avoid inhalation, contact with eyes, skin and clothing. Avoid the formation of dust and aerosols. Use in a wellventilated area. Keep away from sources of ignition. Avoid prolonged or repeated exposure.
UN #'S Not Applicable
Packing Group Not Applicable
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