Leupeptin TFA

  • CAS:147385-61-3
  • Formula:C20H38N6O4 • C2HF3O2
  • MW:540.6 Da
  • Appearance:White solid
  • Purity:>95% by HPLC
Product Name Qty
Leupeptin TFA
5 mg
Leupeptin TFA
10 mg
Leupeptin TFA
25 mg
Leupeptin TFA
50 mg
Leupeptin TFA
100 mg

Leupeptin is a reversible competitive inhibitor of serine and thiol proteases. It has been reported to inhibit calpain, cathepsin B, cathepsins H and L and trypsin . A typical working concentrations is in the range of 10 to 100 µM. Leupeptin appears to be equally effective in any salt form, adjusting for equivalent peptide content. The hemisulfate salts were the first to be commercially available. Of the three salts, the hydrochloride is the least invasive form in biological settings. No problem or preference for the trifluoroacetate (TFA) form has been notes; TFA is volatile, so could possibly be removed by lyophilization.

Microbially produced leupeptin inhibitor was first isolated as a mixture of two very similar forms: acetyl-LeuLeu-Arg-al and propionyl-Leu-Leu-Arg-al. Although the propionyl leupeptin is active as an inhibitor the acetyl form is more commonly used and is available in several different forms.

Leupeptin, because of its aldehyde group, may act as a reducing agent and therefore interfere in protein determinations such as Lowry and, to a lesser extent, Bradford The activity of leupeptins and related analogs has been studied.

Leupeptin stock (20 mg/ml): Leupeptin in Methanol. Prepare a stock of 20 mg/ml in methanol, Store at -20°C.



A potent, reversible inhibitor of serine and cysteine proteases. It showed little to no inhibition activity against: pepsin, cathepsins A and D and alpha-chymotrypsin. All three salt forms are equally effective, when adjusted to their molarity.


Target: Calpain, Plasmin, Trypsin, Papain, Cathepsin B, H, L, Kallikrein, Thrombin,
More Information
Alternate Name/Synonyms
Leupeptin trifluoroacetate salt; Leupeptin 2,2,2-trifluoroacetate; Acetyl-Leu-Leu-Arg-al trifluoroacetate salt; Acetyl-Leu-Leu-Arg-al TFA
Chemical Name
2-acetamido-N-[1-[[5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]-4-methylpentanamide;2,2,2-trifluoroacetic acid
Chemical Formula
C20H38N6O4 • C2HF3O2
Molecular Weight
540.6 Da
White solid
>95% by HPLC
50 mg/mL in water
Stock solution stored in aliquots stable for 2 months.
Storage Temp
Provide appropriate exhaust ventilation at places where dust is formed.
HandlingProvide appropriate exhaust ventilation at places where dust is formed.
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