Miller's LB Broth
91079-40-2; 7647-14-5; 8013-01-2; 77-86-1; 11185-53-1
Water
Room Temperature
Luria Broth is now available in convenient pre-measured and pre-mixed capsules. Prebuffered media eliminates messy weighing and pH adjustments. Molecular biology grade.
For maintenance and propagation of E. Coli.
Protocol:
Simply dissolve 25 capsules per 1 Liter of distilled water. Water must be 37°C or warmer to completely dissolve gelatin capsule. Agitate. Sterilize by autoclaving for 15 minutes before use. Buffer is set for 7.0 ± 0.2 at 25°C using a 10mM solution of Tris/Tris HCl. pH will vary depending on temperature.
Specifications:
- Harmonized Code: 3504.00.10
- pH (2.5 % Solution @25 oC): 6.8 - 7.2
Ingredients:
- Tryptone: 10 g/L
- Sodium Chloride: 10 g/L
- Yeast Extract: 5 g/L
- Tris/Tris HCl: 1.5 g/L
0.1 lbs
Research or further manufacturing use only, not for food or drug use.
