SDS
CofA
Description
Luria Broth is now available in convenient pre-measured and pre-mixed capsules. Prebuffered media eliminates messy weighing and pH adjustments.
For maintenance and propagation of E. Coli. Molecular Biology Grade.
Protocol
Simply dissolve 1 capsule per 1 Liter of distilled water. Water must be 37°C or warmer to completely dissolve gelatin capsule. Agitate. Sterilize by autoclaving for 15 minutes before use. Buffer is set for 7.2 at 20°C using a 10mM solution of Tris/Tris HCl. pH will vary depending on temperature.
Specifications
• Harmonized Code: 3504.00.10
• pH (2.5 % Solution @25 oC): 6.8 - 7.2
• High salt formula
Ingredients
• Tryptone: 10 g/L
• Sodium Chloride: 10 g/L
• Yeast Extract: 5 g/L
• Tris/Tris HCl: 1.5 g/L
More Information
Alternate Name/Synonyms
Miller's LB broth; Luria broth; Lysogeny broth; Lennox broth; Luria-Bertani medium; LB broth; LB medium
SKU
L-2598
CAS #
91079-40-2; 8013-01-2; 7647-14-5; 77-86-1; 1185-53-1
Appearance
Capsules (cream to pale beige free flowing powder)
Solubility
Water
Storage Temp
Room temperature
USE
For maintenance and propagation of Escherichia coli in molecular biology procedures.
Handling
Use in a chemical fume hood, with air supplied by an independent system. Avoid inhalation, contact with eyes, skin and clothing. Avoid the formation of dust and aerosols. Use in a well-ventilated area. Keep away from sources of ignition. Avoid prolonged or repeated exposure.
Certificate of Analysis 1
Certificate of Analysis 2
Certificate of Analysis 3
| GHS PICTOGRAMS |  |
|---|---|
| Handling | Use in a chemical fume hood, with air supplied by an independent system. Avoid inhalation, contact with eyes, skin and clothing. Avoid the formation of dust and aerosols. Use in a well-ventilated area. Keep away from sources of ignition. Avoid prolonged or repeated exposure. |
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