Lysozyme, Purified, Salt-Free

SKU
L-2605
  • CAS:12650-88-3
  • Formula:C125H196N40O36S2
  • MW:14.388 kDa
  • Appearance:Lyophilized powder

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Product Name Qty
Lysozyme from Chicken Egg White, Salt Free
1 g
$46.13
Lysozyme from Chicken Egg White, Salt Free
5 g
$138.38

Description

Lysozyme (salt-free) is an enzyme with antimicrobial properties. Purified from chicken egg-white (Lysozyme C), it hydrolyzes Beta-1,4 glycosidic links between N-acetylmuramic acid and N-acetylglucosamine residues in mucopeptide cell wall structures found in some microbe species, including Micrococcus lysodeikticus. Lysozyme also displays lesser activity toward chitin oligomers. This enzyme exhibits optimal activity at pH 9.2, and is inhibited by surfactants, including dodecyl sulfate, alcohol, and fatty acids.

Lysozyme hydrolyzes Gram-positive at a greater rate due to the higher amounts of peptidoglycan within the cell walls.  Gram-negative cells are less vulnerable to hydrolysis by lysozyme due to the presence of an outer membrane as well as lower amounts of peptidoglycan within the cell walls.

Lysozyme is found in a variety of natural sources, ranging from chicken egg-white, mammalian tissues, saliva, kidneys and more.

 

Applications

• Degrades bacterial cell walls
• Bacterial DNA extractions
• Spheroplast preparation
• MALDI-TOF mass analysis external standard
• Preservative

 

Specifications

• ≥8,000 units per mg dry weight (Technical Note: Due to assay differences, 8,000u/mg by AG’s assay is equivalent to 50,000u/mg claimed by other suppliers.)
• EC Number: 235-747-3

 

More Information
Alternate Name/Synonyms
Muramidase, N-acetylmuramide glycanhydrolase
SKU
L-2605
CAS #
12650-88-3
Chemical Name
(4S)-5-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-6-amino-1-[[(2S)-1-[[(2S)-1-[[2-[[(2S)-1-[[(2S)-1-[[(2S)-4-amino-1-[[(2S)-1-[[(2S)-1-[[2-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[2-[[(1S)-3-amino-1-carboxy-3-oxopropyl]amino]-2-oxoethyl]amino]-4-methyl-1-oxo
Chemical Formula
C125H196N40O36S2
Molecular Weight
14.388 kDa
Appearance
Lyophilized powder
Solubility
Water
Stability
Stable for 3-5 years at 2-8°C. Solutions at pH 4-5 are stable for several weeks refrigerated and for days at ambient temperatures. Store at 2-8°C.
Storage Temp
2-8°C
THERAPEUTIC AREA
Not Available
USE
Lysozyme, Purified, Salt-Free is used for the extraction of genomic DNA from bacterial cells. It is also used as an external standard for MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass analysis.
INCHI
JFXJPYIEDZSWNF-JWBGUOTLSA-N
SMILES
CC(C)CC(C(=O)NC(CC(=O)O)C(=O)NC(CC(=O)N)C(=O)NC(CC1=CC=C(C=C1)O)C(=O)NC(CCCNC(=N)N)C(=O)NCC(=O)NC(CC2=CC=C(C=C2)O)C(=O)NC(CO)C(=O)NC(CC(C)C)C(=O)NCC(=O)NC(CC(=O)N)C(=O)O)NC(=O)CNC(=O)C(CC3=CN=CN3)NC(=O)C(CCCNC(=N)N)NC(=O)C(CCCCN)NC(=O)C(CCSC)NC(=O)C(C)NC(=O)C(C)NC(=O)C(C)NC(=O)C(CC(C)C)NC(=O)C(CCC(=O)O)NC(=O)C(CS)NC(=O)C(CCCNC(=N)N)NC(=O)CNC(=O)C(CC4=CC=CC=C4)NC(=O)C(C(C)C)N
Handling
Ensure adequate ventilation.
Certificate of Analysis 1
GHS PICTOGRAMS
Handling Ensure adequate ventilation.
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