Lyticase 100T, Yeast Lytic Enzyme

SKU
L-5100
  • CAS:37340-57-1
  • Appearance:Lyophilized powder

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Starting at $199.88

Product Name Qty
Lyticase 100T, Yeast Lytic Enzyme
250 mg
$707.25
Lyticase 100T, Yeast Lytic Enzyme
25 mg
$199.88
Lyticase 100T, Yeast Lytic Enzyme
500 mg
$1,086.50
Lyticase 100T, Yeast Lytic Enzyme
50 mg
$287.00
Lyticase 100T, Yeast Lytic Enzyme
100 mg
$420.25

Lyticase, produced by a submerged culture of Arthrobacter luteus, is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by lyticase varies with yeast strain, growth stage of yeast and cultural condition.

Optimum pH for cell wall lysis: 7.5, 35ºC

Optimum pH for yeast glucan hydrolysis: 6.5, 45ºC

Stable pH: 5-10

Activity: ≥100 U/mg

Activators: SH compound such as cystein, 2-mercaptoethanol of dithiothreitol

 
 

*Developed and manufactured by US Biological under contractual agreement for AG Scientific, Inc.

More Information
Alternate Name/Synonyms
Lyticase, Zymolyase, (beta-1,3-glucan laminaripentaohydrolase), Yeast Lytic Enzyme
SKU
L-5100
CAS #
37340-57-1
Appearance
Lyophilized powder
Stability
The lytic activity is lost on incubation at 60°C for 5 minutes.
Storage Temp
4°C
USE
Protoplast/Spheroplast Preparation, Yeast Cell Fusion, Yeast Cell Transformation
Handling
May cause allergy or asthma symptoms or breathing difficulties if inhaled. Avoid inhalation and contact with eyes and skin. Avoid prolonged or repeated exposure.
Hazard Image
Yes
Shelf Life
Stable for 1 year at 4ºC. About 90% of the lytic activity is lost when stored at 30ºC for 3 months.
GHS PICTOGRAMS
Handling May cause allergy or asthma symptoms or breathing difficulties if inhaled. Avoid inhalation and contact with eyes and skin. Avoid prolonged or repeated exposure.
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