2-8°C
NHS-Activated Agarose 4B-CL (With Spacer Arm) aids with the rapid immobilization of antibodies and other proteins to the matrix. N-hydroxyl succinimide (NHS) ester forms a stable amide (peptide) bond with the primary amines of ligands. NHS-Activated Agarose provides a valuable tool for affinity purification of antibodies, antigens and other biomolecules.
N-hydroxyl succinimide (NHS) ester forms a stable amide (peptide) bond with the primary amines of ligands. The stability of an amide bond at high pH (up to pH 13) allows for a very stringent wash protocol during affinity purification using NHS-activated ligand coupling.
Coupling can be carried out in neutral pH which makes it ideal for immobilization of antibodies, antigens, and other proteins sensitive to pH extremes. Coupling reaction can also be carried out in organic solvents.
Provides a 10 atom spacer arm upon ligand coupling. Coupling completed after 2 - 4 hours at room temperature.
Applications:
- Rapid immobilization of antibodies and other proteins to the matrix.
- Immobilization of ligands through primary amines to purify recombinant proteins.
- Immobilization of Protein A or Protein G for monoclonal antibody purification.
- Pre-activated medium for immobilization of ligands containing a primary amino group.
Specifications:
- Matrix: Agarose 4B-CL (highly crosslinked agarose beads, 4%)
- Particle Size Range: 53 - 180 μm
- Spacer Arm: 6-aminocaproic acid, 10 atoms
- Suspension/Column/Catridge: pre-swelled gel suspension in 100% isopropanol to protect the active groups
- pH Stability: 3 - 13 (ligand dependent)
- Flow Specifications: 70 -140 cm/h
- Coupling Conditions: pH 6 - 9; Temp: 4 - 25°C
- Ligand Density: >18 μmol NHS / mL drained gel
- Molecular Weight Range: 6 x 104 - 2 x 107
0.1 lbs
Research or further manufacturing use only, not for food or drug use.
