PRImeDETECT™ Legionella spp Detection Kit (Legionella spp) is an ideal tool for a fast and reliable amplification and detection of specific DNA fragment from Legionella spp by the real-time PCR method.
The kit includes all reagents required in a comfortable ready-to-use PCR MasterMix. The optimized MasterMix contains a buffer, dNTPs, Hot-start DNA Polymerase, DNA-free water, MgCl2 and an internal amplification control (IAC) whose detection indicates the absence of PCR inhibitors.
Primers and probes for the amplification of IAC as well as for the amplification of the target gene are included in the MasterMix. The probe for the detection of target gene is labelled with the FAM (Legionella spp), whereas the probe for the detection of IAC is labelled with the JOE fluorochrome
In addition, the kit includes both positive control and negative control. The positive control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a negative control reaction should be included every time the kit is used. Include DNAready Lysis Buffer to extract the DNA from the sample prior to PCR Detection.
Advantages & Features
• Easy and ready-to-use kit
• Fast: results in less than 24 hours with minimal handling
• Reliable and highly specific results
• Includes materials for 48 rxn: 1 x PCR MasterMix, 1 x DNAready Lysis Buffer, 1 x PCR Positive Control, 1 x PCR Negative Control
• Amplification limit: 2.5 copies/reaction (95%)
• Quantification limit: 5 copies/reaction (95%)
• Quantification dynamic range: 8 logs.
• Target: Legionella spp.
• Sample: environmental and biological samples (water, sediments, sputum, blood, serum, and urine)
• Inclusivity: 100%. Primers and probes have been tested satisfactorily in 29 strains of Legionella
• Exclusivity: 100%. Primers and probes have been tested satisfactorily in 28 non-Legionella bacteria
• Compliance: Manufactured according to ISO/TS 12869:2012.
• Detection: Probe labelled with fluorescent dyes– Legionella: FAM-TAMRA; IAC: JOE-TAMRA.
• Thermal Cycler: Agilent Mx3005P, Applied Biosystems 7300, 7500 and other cyclers
*Developed and manufactured by Canvax Biotechnology, S.L under contractual agreement for AG Scientific, Inc.