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G-418.Sulfate, Sterile-Filtered Solution, 10 ML

Price$ 41.01
  • SKU: G-1035-10ML
  • Pack Size: 10 ML
Quantity
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Total price: $ 162.64

This item: G-418.Sulfate, Sterile-Filtered Solution, 10 ML $ 41.01

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108321-42-2

692.7 Da

C20H40O10N4 • 2H2SO4

Colorless solution (50 mg/mL in H2O)

+4°C


Description

*AG Scientific's G-418 sulfate solution is a sterile filterate solution.*

G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is commonly used in plant and mammalian cell culture as a selectable marker. G418 disulfate is freely soluble in water. Bacteria and algae require 5 µg/ml or less while animal cells may require 300-500 µg/ml.  It functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation.

Resistance to G-418 is conferred through the nptll gene in plant cells and the neo gene in mammalian cells, which encode aminoglycoside 3'-phosphotransferase. For gene selection applications, a kill curve can be made to determine the minimum effective G418 concentration to kill non-resistant cells. G418 longstanding application as a gene selection antibiotic, has a powerful new role in Genome editing utilizing the CRISPR/Cas9 system.

Purity data is for a concentration of 50mg/ml active.

 

Not for human therapeutic use or for medicinal purposes. For research applications only.

0.1 lbs



Research or further manufacturing use only, not for food or drug use.

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