Puromycin Dihydrochloride Solution, 5X2 ML

AG Scientific's Puromycin dihydrochloride solution is a sterile filtrate solution.
 

Puromycin is an aminonucleoside antibiotic that causes early chain termination within the ribosome during translation. This inhibition of translation is effective in both in vivo and in vitro applications, making it an ideal tool for protein synthesis studies. While selection for E. Coli cells is possible with puromycin, it is more commonly and effectively used for mammalian cell selection.
 

It is active against gram-positive microorganisms, less active against acid-fast bacilli and more-weakly active against gram-negative microorganisms. The growth of bacteria, protozoa, algae and mammalian cells is quickly halted by puromycin. However, cells with the pac gene have resistance, its gene product is puromycin-N-acetyl-transferase which inactivates puromycin through acetylation. Research has shown puromycin to be effective against certain tumors.
 

Recently researchers have developed a prodrug strategy for selective cancer therapy using a masked cytotoxic agent Fmoc-Lys(Ac)-Puromycin, which can be sequentially activated by histone deacetylases (HDACs) and cathepsin L (CTSL) to kill cancer cells expressing high levels of both enzymes. The data gathered indicates that Fmoc-Lys(Ac)-Puromycin can substantially improve anti-cancer efficacy and can be developed into potential selective cancer therapy.

Applications:

• Puromycin is soluble in water and is often used as a selective agent in molecular biology research.
• Puromycin has a longstanding application as a gene selection antibiotic and a powerful new role in Genome editing utilizing the CRISPR/Cas9 system.