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AMP(Agarose) 4B-CL (Lyophilized), 1 G

Price$ 401.85
  • SKU: A-2917-1GM
  • Pack Size: 1 G
Quantity
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-20°C


Applications:

AMP interacts strongly with NAD+-dependent dehydrogenases and ATP-dependent enzymes. Selective elution with gradients of NAD+ or NADP+ allows the resolution of complex mixtures of dehydrogenase isoenzymes using 5`-AMP Separopore®.

The production method involves alkylation of the nucleotide, AMP followed by alkaline rearrangement to yield the corresponding N6-carboxymethyl derivative with subsequent condensation using 1,6-diaminohexane to give N6-[(6-aminohexyl)carbamoylmethyl)].

 

Technical Specifications:

  • Ligand: Adenosine Monophosphate (AMP)
  • Matrix: Separopore® 4B-CL (highly crosslinked agarose beads, 4%).
  • Particle size range: 52 – 165 µm
  • Matrix activation: Epoxy
  • Matrix attachment: N6
  • Spacer arm: 1, 6-diaminohexane
  • Ligand density: 2 µmol AMP / ml drained gel
  • Binding capacity: ~ 5-7 mg lactate dehydrogenase / ml drained gel
  • pH stability: 4 – 10
  • Flow rate specifications: 70 – 140 cm / h.
  • Chemical stability: Stable to all commonly used aqueous buffers and additives like detergents. Avoid high concentrations of EDTA, urea, guanidine-HCl, chaotropic salts and strong oxidizing agents
  • Physical stability: Negligible volume variation due to changes in pH or ionic strength
  • Supplied as lyophilized powder and 1g yields 8-10 ml of gel

0.1 lbs



Research or further manufacturing use only, not for food or drug use.

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