2-8°C
Applications:
- Rapid immobilization of antibodies and other proteins to the matrix.
- Immobilization of ligands through primary amines to purify recombinant proteins.
- Immobilization of Protein A or Protein G for monoclonal antibody purification.
- Pre-activated medium for immobilization of ligands containing a primary amino group.
- N-hydroxyl succinimide (NHS) ester forms a stable amide (peptide) bond with the primary amines of ligands.
- The stability of an amide bond at high pH (up to pH 13) allows for a very stringent wash protocol during affinity purification using NHS-activated ligand coupling.
- Coupling can be carried out in neutral pH which makes it ideal for immobilization of antibodies, antigens, and other proteins sensitive to pH extremes.
- Coupling reaction can also be carried out in organic solvents.
- Provides a 10 atom spacer arm upon ligand coupling.
- Coupling completed after 2 – 4 h at room temperature.
- NHS-Activated 6B-CL provides a valuable tool for affinity purification of antibodies, antigens and other biomolecules.
Technical specifications:
- Group to be coupled: Primary -NH2
- Matrix: 6B-CL (highly crosslinked agarose beads, 6%)
- Particle size range: 52 – 165 μm
- Molecular weight range: 6 x 104 – 2 x 107
- Chemical linkage of ligand: Amide bond
- Spacer arm: 6-aminocaproic acid, 10 atoms
- Substitution: >18 μmol NHS / ml drained gel
- pH stability: 3 – 13 (ligand dependent)
- Coupling conditions: pH 6 – 9; Temp: 4 – 25 °C
- Storage: 2 – 8 °C
- Flow rate specifications: 70 – 140 cm / h
- Supplied as a pre-swelled gel suspension in 100% isopropanol to protect the active groups
0.1 lbs
Research or further manufacturing use only, not for food or drug use.