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Ni2+-Chelated Agarose 4B-CL, 10 ML

Price$ 197.44
  • SKU: N-2749-10ML
  • Pack Size: 10 ML
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Total price: $ 347.44

This item: Ni2+-Chelated Agarose 4B-CL, 10 ML $ 197.44

GTP Agarose 4B-CL, 1 ML $ 150.00

+4°C


Applications:

Ni2+-chelated 4B-CL is an imminodiacetate (IDA)-immobilized metal affinity chromatography (IMAC) matrix that separates proteins via their differences in affinity to nickel metal ions. Ni2+-chelated 4B-CL is ideal for purification of recombinant proteins containing (His)6 fusion tag.

 

Technical Specifications:

  • Chelating group: Nickel
  • Matrix: 4B-CL (crosslinked agarose beads, 4%)
  • Matrix activation: Epoxy
  • Particle size range: 52 – 165 µm
  • Metal ion capacity: >20µmol Ni2+ / ml drained gel
  • Binding capacity: ~5 – 8 mg (His)6-tagged protein / ml medium
  • pH stability: 2 – 11
  • Chemical stability: For one week at 40 °C: 0.01M HCl, 0.1M NaOH

        For 12 h: 1M NaOH, 70% acetic acid.

       For 30 min tested: 30% 2-propanol.

        For 1 h tested: 2% SDS 

  • Compatibility during use: Stable in all commonly used buffers, reducing agents, denaturants, and detergents
  • Supplied as suspension in 20% ethanol

 

0.1 lbs



Research or further manufacturing use only, not for food or drug use.

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