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Price$ 131.96
You Save$ 1118.04
  • SKU: X-1015-5GM
  • Pack Size: 5G
Quantity
-
+

7240-90-6

408.61

C14H15BrClNO6

Soluble 2% in DMF

-20°C


Description

X-Gal, formally referred to as 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, is commonly used in molecular biology in regards to cloning applications. First synthesized in 1964, this organic compound contains galactose, which is also found in casein, yogurt, and milk, as well as certain dairy-free products.

An analog of lactose, X-Gal is hydrolyzed by the β-galactosidase enzyme, resulting in 5,5'-dibromo-4,4'-dichloro-indigo -- an insoluble blue precipitate. This indicates enzymatic activity. For example, X-Gal can be used in molecular biology to detect genetic activity in cells and tissues.

 

Applications

Commonly used in blue/white screening, X-Gal yields a visual indication regarding the cellular expression of a functional β-galactosidase enzyme. In turn, key recombinant colonies can be easily identified and then cultured.

 

Not for human therapeutic use or for medicinal purposes. For research applications only.

0.1 lbs



Research or further manufacturing use only, not for food or drug use.

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