3483-12-3
154.2
C4H10O2S2
10% solution in water. Clear and colorless
+4°C
Description
Dithiothreitol (DTT), is a small-molecule redox reagent, known as Cleland's reagent, that forms a six-member ring with an internal disulfide bond. It has a redox potential of -0.33 V at pH 7. The reduction of typical disulfide bond proceeds by two sequential thiol-disulfide exchange reactions.
DTT is effective in buffers to reduce protein disulfide bonds prior to SDS-PAGE. It can protect protein sulfhydryls and restore enzyme activity lost by oxidation.
Applications
• For reducing or "deprotecting" agent for thiolated DNA
• For reducing disulfide bonds of proteins
• Used as an oxidizing agent
• Used as a component of medium for the demembranation and reactivation of spermatozoa
• Used to prevent intramolecular and intermolecular disulfide bonds from forming between cysteine residues of proteins
Additional Reading from AG Scientific
• DTT (Dithiothreitol): Frequently Asked Questions
• DTT (Dithiothreitol): Applications You Must Know
• TCEP HCl vs. DTT: Preferred Reducing Agents
• Download Brochure: Reducing Agents
Not for human therapeutic use or for medicinal purposes. For research applications only.
0.1 lbs
Research or further manufacturing use only, not for food or drug use.
