7732-18-5; 77-86-1; 5704-04-1; 151-21-3
TRIS-Tricine-SDS running buffer is used in the Schagger and Von Jagow method. This buffer is used to separate low molecular weight proteins and peptides. Peptide-SDS complexes move more slowly through Tris-tricine gels than they do through Tris-glycine buffers, allowing for the complete separation of SDS micelles that otherwise normally interfere with peptide separations. The tricine allows for efficient stacking and de-stacking of low molecular weight peptides, resulting in higher resolution of smaller peptides.
• TRIS: 1 M
• Tricene: 1 M
• SDS (pH 8.3): 1%
• pH: 8.1-8.5
Research or further manufacturing use only, not for food or drug use.