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Decontaminase™, 25 KU

Price$ 238.24
  • SKU: B-1400-25KU
  • Pack Size: 25 KU
Quantity
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Binuclease Endonuclease; Serratia marcescens binuclease; Serratia marcescens nuclease; unrestricted endonuclease; Benzonase Nuclease

9025-65-4

Glycerol

-20°C


Description:

Decontaminase™ (binuclease endonuclease) is synthesized from yeast cells with cloned gene encoding genetically engineered Serratia marcescens endonuclease. Decontaminase™ is an ideal alternative to Benzonase. It digests all forms of DNA and RNA, including single and double-stranded, linear, circular, and supercoiled.


Decontaminase™ cleaves phosphodiester bond of nucleic acids. Produces 5' monophosphate terminated oligonucleotides 2-5 bases in length.

 

Applications:

  • Removes nucleic acids in biological products
  • Reduces viscosity in cell lysates
  • Pharmaceutical industry: eliminate nucleic acid contamination in vaccines and proteins
  • Bioanalytical applications: ELISA sample preparation, chromatography, two-dimensional electrophoresis (protein mapping), and footprint analysis for improved resolution and increased sample recovery.

 

Specifications:

  • E.C.: 3.1.30.2
  • pH: 6-10, optimum pH 8.0
  • Optimum temperature: 37ºC
  • Temperature range of enzyme activity: 0-42ºC
  • Specific activity: ≥ 1,000 kU / mg protein
  • No products of animal origin used, no viral contamination
  • Endotoxin free and protease free. Stable under harsh industrial conditions.
  • Activators: 1~10 mM Mg2+

 

Unit Definition:

One unit of Decontaminase™ will digest sonicated salmon sperm DNA to produce acid-soluble oligonucleotides equivalent to a ΔA260nm of 1.0 in 30 minutes at pH 8.0 at 37ºC.

 

Activity:

  • ≥ 20 kU/mg lyophilized powder
  • ≥ 250 U/μL buffered aqueous glycerol solution

 
 

0.1 lbs



Research or further manufacturing use only, not for food or drug use.