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Diaphorase NADH, 1 KU

Price$ 395.58
  • SKU: N-1181-1KU
  • Pack Size: 1 KU
Quantity
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Total price: $ 798.75

This item: Diaphorase NADH, 1 KU $ 395.58

Diaphorase NADPH, 1 KU $ 403.17

Diaphorase NADH, 5 KU $ 0.00

9032-20-6

50 kDa (SDS-PAGE), 160 kDa (gel filtration)

Soluble in water.

-20°C


Description:

Diaphorase are dehydrogenase enzymes which catalyze the dehydrogenation of the reduced forms of the co-enzyme NADH and NADPH. NADH and NADPH originally come from purified extracts of Clostridium kluyveri cells, where diaphorase activity is shown. Other diaphorase activities come from various bacteria, plants and mammalian organs. This enzyme was isolated from Bacillus megaterium.

 

Applications:

• Used in the reoxidation of NADP to NADPH
• Used to construct a biosensor for acetaldehyde by immobilization
• Used in a study to assess the protein-protein interactions in assembly of lipoic acid on the 2-oxoacid dehydrogenases of aerobic metabolism

 

Specifications

• EC 1.6.5.2
• More than 70 U/mg solid
• Isoelectric point: pH 4.2
• Michaelis constant: NADH 5.5x10^-4 M
• Optimum pH: 8.0-9.0
• pH stability: 6.0-9.0 (37 C, 60 min)
• Optimum temperature: 40-45 C
• Thermal Stability: stable at 50 C and below (pH 8.0, 10 min)

 

Not for human therapeutic use or for medicinal purposes. For research applications only.

0.1 lbs



Research or further manufacturing use only, not for food or drug use.

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