Lyticase, produced by a submerged culture of Arthrobacter luteus, is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase.
It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis.
The extent of lysis of yeast cells by lyticase varies with yeast strain, growth stage of yeast and cultural condition.
• Optimum pH for cell wall lysis: 7.5, 35ºC
• Optimum pH for yeast glucan hydrolysis: 6.5, 45ºC
• Stable pH: 5-10
• Activity: ≥20 U/mg
• Activators: SH compound such as cystein, 2-mercaptoethanol or dithiothreitol
*Developed and manufactured by US Biological under contractual agreement for AG Scientific, Inc.
Research or further manufacturing use only, not for food or drug use.