DISCONTINUED Proteinase K

This product has been discontinued.

Please see Proteinase K, recombinant (P-1264)

 

Description

Proteinase K (also known as endopeptidase K) is an extracellular endopeptidase isolated from a culture filtrate of the fungus Tritirachium album limber. This unique fungus can degrade keratin (hence Proteinase “K”) and can grow using the by-product as its sole source of carbon and nitrogen.

The enzyme is stable over a broad temperature and pH range, with optimum activity at 55-65°C and pH 8.0. It’s also stable in high salt concentrations and active in the presence of strong detergents such as SDS. It has a molecular weight of 28.5 kDa and it shows fairly-low specificity for its polypeptide targets.

 

Features and Benefits of AG’s Proteinase K

• DNAse and RNAse free via chromatography purification
• 6x greater activity than Pronase and 3x greater activity than bovine trypsin
• Broad substrate specificity
• Versatile in blood, cultured cells, tissues, bacteria, yeast, etc.
• Active and stable over a wide range of conditions
• Compatible with most DNA and RNA isolation buffers

 

Applications in Next Generation Sequencing (NGS) and Microarray Technologies

• Nucleic acid purification by inactivating nucleases when extracting DNA and RNA from yeast, bacteria, and mammalian cell & plant cell lysates
• Improves cloning efficiency of PCR products
• Sample preparation for quantification of DNA adduct levels by accelerator mass spectrometry
• Inactivation of enzyme cocktails in ribonuclease protection assays
• Added to extraction procedures to optimize RNA yields from primary breast tumors for microarray studies

 

Applications with Proteins

• Detection of Bovine Spongiform Encephalopathy proteins which are uniquely resistant to proteolytic degradation.
• Tissue digestion as an alternative sample preparation approach for quantitative analysis using liquid chromatography—tandem mass spectrometry
• Specifically modify cell surface proteins to analyze membrane structures for protein localization
• Generates protein fragments used in characterization of functional studies

 

Specifications

• EC Number: 3.4.21.64 

 

Additional Reading from AG Scientific