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M-MLV Reverse Transcriptase, 10,000 UNITS

Price$ 95.00
  • SKU: M-3337-10000U
  • Pack Size: 10,000 UNITS
Quantity
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69 kDa

-20°C


Description

M-MLV Reverse Transcriptase (MMLV-RT), encoded by Moloney Murine Leukemia Virus (MMLV) is an RNA-dependent DNA polymerase that synthesizes the cDNA first-strand from a single-stranded RNA template to which a primer has been hybridized. MMLV-RT will also extend primers hybridized to single-stranded DNA.

Second-strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase.

 

Advantages & Features

• Concentration: 200 U/µL
• Full Solution: includes reaction buffer 5x
• High Quality: free of DNase, RNase or protease contamination
• Purity: >90% as judged by SDS-polyacrylamide gels with blue staining
• Final Product Size: 7 kb or less

 

Applications

• RT PCR
• Synthesis of cDNA
• mRNA 5’-end Mapping by Primer Extension Analysis
• End-labeling of DNA
• Dideoxynucleotide Sequencing

 

Specifications

• Source: purified from E.coli strain harboring a plasmid that directs the synthesis of modified form of MMLV-RT
• One unit of the enzyme incorporates 1 nmol dTTP into acid-precipitable material in 10 minutes at 37 ° C, using poly(A) oligo dT as a template primer
• Exempt of nucleases (endo, exo and ribonucleases) activities
• Purity: >90% as judged by SDS-polyacrylamide gels with blue staining

 

*Developed and manufactured by Canvax Biotechnology, S.L under contractual agreement for AG Scientific, Inc.

0.1 lbs



Research or further manufacturing use only, not for food or drug use.

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