Quick Order Pad
Your Shopping Cart is Empty
Hello, Sign In

Pyruvate Oxidase, 5 KU

Price$ 750.00
  • SKU: P-1600-5KU
  • Pack Size: 5 KU
Quantity
-
+

9001-96-1

Not Available

-20°C


Description

Pyruvate oxidase (E.C. 1.2.3.3) is originated from Aerococcus viridans and used as a component of screen-printed phosphate biosensor for monitoring phosphate concentrations in a sequencing batch reactor (SBR) system. It consumes phosphate in the presence of pyruvate and oxygen and generates hydrogen peroxide, carbon dioxide and acetylphosphate.

The enzyme is a flavoprotein with the relatively tightly bound cofactors flavin adenine dinucleotide, thiamine pyrophosphate, and a divalent metal ion, with Mn2+ being the most effective.

Activity: 40 units/mg powder. One unit is defined as amount of enzyme required to generate 1umol of H202 per minute at 37C, pH 6.5. Contaminate info: Glutamate oxalacetate transaminase (GOT) 002% by activity; Glutamate pyruvate transaminase (GPT) 006% by activity; Lactate Oxidase 002% by activity; Catalase not detectable.

 

Applications

• Study of aerobic metabolism of bacteria

• Used for enzymatic determination of AST and ALT

• Used to monitor phosphate concentration in SBR system

 

Additional Reading

• Electrochemical Processes for Treating Water and Why it Matters

• Single-Use Biosensors for the Detection of Biomarkers

• Advances in Lab-on-a-Chip Technology

 

References

• “Purification and biochemical characterization of pyruvate oxidase from Lactobacillus plantarum”

• “A screen-printed biosensor using pyruvate oxidase for rapid determination of phosphate in synthetic wastewater”

• “Role of the divalent metal cation in the pyruvate oxidase reaction.”

 

Not for human therapeutic use or for medicinal purposes. For research applications only.

0.1 lbs



Research or further manufacturing use only, not for food or drug use.

Product Recommendations