Protease Inhibitor Cocktail III, Animal-Free, DMSO-Free is a specially formulated mixture of six protease inhibitors with broad specificity for the inhibition of aspartic, cysteine and serine proteases as well as aminopeptidases.
For applications that require animal-free reagents and where the use of DMSO is not recommended. 1 vial is recommended for the inhibition of proteases extracted from 20 g of tissue.
When reconstituted to 1 mL, each vial contains:
- 100 mM AEBSF HCl
- 80 μM Aprotinin, recombinant (animal-free)
- 5 mM Bestatin
- 1.5 mM E-64
- 2 mM Leupeptin hemisulfate
- 1 mM Pepstatin A
Preparation: Add directly to extraction media or mix with DMSO, water, or aqueous buffers.
Research or further manufacturing use only, not for food or drug use.