qMAXSen™ One-Step Probe RT-qPCR Kit (Low ROX™)

SKU
Q-2451

Starting at $249.99

Product Name Qty
qMAXSen™ One-Step Probe RT-qPCR Kit (Low Rox™)
100 rxn
$249.99

Description

qMAXSen™ One-Step Probe RT-qPCR Kit (Low ROX™) offers greater specificity, sensitivity, and yields in cDNA synthesis and qPCR in a single tube. The kit includes a qPCR MasterMix supplied in a 2x concentration to perform Real-Time PCR. The qPCR MasterMix contains all the reagent (except PCR primers, probe and template) needed for running PCR reactions. The mix is compatible with many probe technologies. In addition, a separate RT mix that comprises a balanced mixture of both RTase and RNase Inhibitor is also supplied.

Available with the option of ROX™ as the internal passive reference dye. The ROX™ dye provides an internal reference to which the reporter-dye signal can be normalized during data analysis. See related product: Q-2453 (High ROX™).

RT-PCR is used to amplify double-stranded DNA from single-stranded RNA templates. In the RT step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR step synthesis, Taq DNA polymerase synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. During subsequent rounds of cycling the DNA polymerase exponentially amplifies the double-stranded DNA template.

 

Advantages & Features

• Enhanced specificity, sensitivity, and yields in cDNA synthesis
• Versatile: compatible with most used real-time PCR instruments
• Compatible with a wide range of probe technologies including Taqman®, Molecular Beacons®, and Scorpion® probes
• Convenient: includes internal passive reference dye
• Easy reaction assembly

 

Applications

• One step qRT-PCR based on specific probes
• Detection and quantification of DNA and cDNA targets
• Gene expression
• Standard and fast qPCR platforms
• High throughput applications

 

Specifications

• Includes materials for 100 rxn: 1 mL qMAXSen™ One-Step RT-qPCR (2x), 100 μL RT mix, 1 mL RNase-free water

 

*Developed and manufactured by Canvax Biotechnology, S.L under contractual agreement for AG Scientific, Inc.

More Information
SKU
Q-2451
Storage Temp
Upon receipt of the kit, immediately store the components at -20 °C in a freezer without a defrost cycle. It is recommended to avoid repeated freezing and thawing.
USE
qRT-PCR, detection and quantification of DNA and cDNA, cDNA synthesis, gene expression, high throughput applications
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