RIPA Buffer 2X Solution

SKU
R-2417
  • CAS:7732-18-5; 1185-53-1; 7647-14-5; 127087-87-0; 302-95-4; 151-21-3
  • Appearance:Clear solution

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Product Name Qty
RIPA Buffer
125 mL
$77.00
RIPA Buffer
250 mL
$109.00

Description

RIPA (Radio-Immunoprecipitation assay) buffer is used to lyse cells and tissue, for radio immunoprecipitation assay (RIPA). RIPA lysis buffer has stronger denaturing capabilities than NP-40 or Triton X-100 and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts. RIPA buffer also results in low background in immunoprecipitation/pull down assays.

 

More Information
Alternate Name/Synonyms
Radio-Immunoprecipitation Assay Buffer, RIPA
SKU
R-2417
CAS #
7732-18-5; 1185-53-1; 7647-14-5; 127087-87-0; 302-95-4; 151-21-3
Chemical Name
α-[(1,1,3,3-Tetramethylbutyl)phenyl]-ω-hydroxy-poly(oxy-1,2-ethanediyl)
Appearance
Clear solution
Solubility
Water
Melting Point
N/A
Preparation
N/A
Storage Temp
2-8°C
THERAPEUTIC AREA
Not Applicable
USE
RIPA buffer is useful for lysing and extracting protein from cultured mammalian cells, including plated cells and pelleted suspencsion cells for radioimmunoprecipitation assay. This buffer is more denaturing than NP-40 or Triton X-100 because it contains the ionic detergents SDS and sodium deoxycholate as active constituents and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts. The RIPA buffer gives low background but can denature kinases.
Merck Index
N/A
MDL NUMBER
MFCD02100484
UN #'S
UN 2928
PACKING GROUP
II
Handling
Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
GHS PICTOGRAMS
Handling Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
UN #'S UN 2928
Packing Group II
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