Streptavidin (Agarose) 4B

SKU
S-2913
  • CAS:9013-20-1; 9012-36-6
  • Appearance:Liquid suspension
  • Purity:>96%
Product Name Qty
Streptavidin (Agarose) 4B
1 ml
$144.99
Streptavidin (Agarose) 4B
5 ml
$443.80
Streptavidin (Agarose) 4B
10 ml
$844.48

Applications:

  • Affinity matrix with immobilized Streptavidin by CNBr activation coupling method.
  • The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability.
  • The immobilized streptavidin binds biotin and biotinylated substances and can be used for affinity chromatography applications.
  • The interaction between streptavidin and biotin is very strong and requires denaturating conditions for elution, which may destroy both the ligandand the sample.
  • Strptavidin- 4B is extremely useful for exploiting either the strong interactions of biotin and streptavidin or the somewhat weaker interactions of 2-iminobiotin and streptavidin.
  • It can be used in the purification of antigens, where biotinylated antibodies are incubated with antigen. The biotinylated antibody-antigen complex binds to Streptavidin Separopore from which the antigen can be eluted.

 

Technical specifications

  • Ligand: Native Streptavidin (from Streptomyces avidinii)
  • Matrix: agarose beads, 4%
  • Particle size range: 52 -165 µm
  • Matrix activation: Cyanogen bromide
  • Ligand density: 2 mg streptavidin / ml drained gel
  • Binding capacity: >20 µg biotin / ml drained gel
  • Exclusion limit: ~ 2 x 107
  • pH stability: 3 – 10
  • Binding buffer: 50 mM ammonium carbonate, 0.5M NaCl, pH 10
  • Elution buffer: 50 mM ammonium acetate, 0.5M NaCl, pH 4
  • Supplied as suspension in buffered saline solution.
More Information
SKU
S-2913
CAS #
9013-20-1; 9012-36-6
Chemical Name
Sodium azide
Appearance
Liquid suspension
Purity
>96%
Storage Temp
4°C
THERAPEUTIC AREA
Not Applicable
USE
Streptavidin (Agarose) 4B is used in purification of membrane antigens in conjunction with biotinylated monoclonal antibodies, and DNA fingerprinting with streptavidin-coated plates. It is also applied to the isolation of cell-surface proteins that have been labeled with a cleavable biotinylation reagent, and to the isolation of cell-surface glycoproteins using biotinylated Concanavalin A.
Handling
Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
HandlingUse only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
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