Applications:

• Affinity matrix with immobilized Streptavidin by CNBr activation coupling method.
• The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability.
• The immobilized streptavidin binds biotin and biotinylated substances and can be used for affinity chromatography applications.
• The interaction between streptavidin and biotin is very strong and requires denaturating conditions for elution, which may destroy both the ligandand the sample.
• Strptavidin- 4B is extremely useful for exploiting either the strong interactions of biotin and streptavidin or the somewhat weaker interactions of 2-iminobiotin and streptavidin.
• It can be used in the purification of antigens, where biotinylated antibodies are incubated with antigen. The biotinylated antibody-antigen complex binds to Streptavidin Separopore from which the antigen can be eluted.

Technical specifications

• Ligand: Native Streptavidin (from Streptomyces avidinii)
• Matrix: agarose beads, 4%
• Particle size range: 52 -165 µm
• Matrix activation: Cyanogen bromide
• Ligand density: 2 mg streptavidin / ml drained gel
• Binding capacity: >20 µg biotin / ml drained gel
• Exclusion limit: ~ 2 x 10⁷
• pH stability: 3 – 10
• Binding buffer: 50 mM ammonium carbonate, 0.5M NaCl, pH 10
• Elution buffer: 50 mM ammonium acetate, 0.5M NaCl, pH 4
• Supplied as suspension in buffered saline solution.