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TBARS Assay Kit, 96 Assays

Price$ 261.25
  • SKU: T-2837-96ASSAYS
  • Pack Size: 96 Assays
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2-8°C


Oxidative stress in the cellular environment results in the formation of highly reactive and unstable lipid hydroperoxides. Decomposition of the unstable peroxides derived from polyunsaturated fatty acids results in the formation of malondialdehyde (MDA), which can be quantified colorimetrically following its controlled reaction with thiobarbituric acid. Thiobarbituric Acid Reactive Substances (TBARS) Assay was proposed over 40 years ago and is now the most commonly used method for screening and monitoring lipid oxidation.
 

TBARS method has been used to evaluate a wide range of samples that include human and animal tissues and fluids, drugs, foods and natural products. The sensitivity of measuring Thiobarbituric Acid Reactive Substances (TBARS) has made this assay the method of choice for screening and monitoring lipid peroxidation, a major indicator of oxidative stress. Even though there remains a controversy cited in the literature regarding the specificity of TBARS toward compounds other than MDA, it still remains the most widely employed assay used to determine lipid peroxidation.
 

This assay is based on the reaction of malondialdehyde (MDA) with thiobarbituric acid (TBA); TBA reacts with MDA to form a pink chromogen, which can be detected spectrophotometrically at 532 nm.


Advantages & Features:

• Accurate
• Simple & Fast Protocol
• Versatile: Colorimetric or Fluorometric MDA Quantitation
• Standardized tool for assaying lipid peroxidation in Plasma, Serum, Urine, Tissue homogenates, and Cell Lysates


Applications:

• For quantitative determination of lipid Peroxides (thiobarbituric acid reactive substances, TBARS)
• Evaluation of drug effects on lipid Peroxidation


Specifications:

• Includes materials for 96 wells: 4 x 0.53 g Thiobarbituric Acid (TBA), 40 mL Diluent 1 (contain Acetic Acid), 20 mL Diluent 2 (contain NaOH), 10 mL SDS Solution, 250 μL MDA Standard (10mM), 20 mL H2O2 solution, 1 unit 96-Well Solid Black plate, 1 unit 96-Well Solid Clear plate, 2 Adhesive strips

 

*Developed and manufactured by Canvax Biotechnology, S.L under contractual agreement for AG Scientific, Inc.

 

0.1 lbs



Research or further manufacturing use only, not for food or drug use.

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