TMB, High Kinetics

SKU
T-2463
  • CAS:54827-17-7
  • Formula:C16H20N2
  • MW:240.35
  • Appearance:Liquid
  • Purity:N/A
Product Name Qty
TMB, High Kinetics
100 mL
$123.02
3,3',5,5'-Tetramethylbenzidine (TMB) has been shown to be a safe, sensitive substrate for the assay of horseradish peroxidase (HRP). Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is formed. This compound, a cation free radical, is blue in color with an adsorption maximum at 653 nm. Further reaction with HRP/H, or acidification of the radical with acid yields the diimine terminal oxidation product adsorbing light at 450 nm. The extinction coefficient of the radical (E653 nm = 3.9 x 10 mol⁻ cm⁻) and diimine (E450 nm = 5.9 x 10 mol⁻ cm⁻ ) provide a remarkably sensitive system for the assay of HRP and HRP labeled probes. TMBHK, available from BioVision Inc., is a highly sensitive single component reagent that is ready to use for the quantitative detection of HRP bound to a solid phase or in free solution. TMBHK is stable at room temperature for six months and is not sensitive to normal laboratory light. It is optimized with respect to TMB and hydrogen peroxide concentrations and yields a linear response with the concentrations of HRP commonly employed in immunologic assays.
More Information
Alternate Name/Synonyms
3,3',5,5'-Tetramethylbenzidine; TMBHK
SKU
T-2463
CAS #
54827-17-7
Chemical Name
3,3',5,5'-Tetramethylbenzidine; TMBHK
Chemical Formula
C16H20N2
Molecular Weight
240.35
Appearance
Liquid
Storage Temp
+4°C
THERAPEUTIC AREA
Not Available
USE
TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays
MDL NUMBER
MFCD00007748
CHEMACX
X1009100-2
INCHI
InChI=1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
SMILES
Cc1cc(cc(c1N)C)c2cc(c(c(c2)C)N)C
Peptide sequence
Not Applicable
RTECS
DV2300000
UN #'S
Not Applicable
PACKING GROUP
Not Applicable
HAZARD CLASS
Not Applicable
Handling
Protect from light
Handling Protect from light
UN #'S Not Applicable
Packing Group Not Applicable
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