TMB, Ultrasensitive

SKU
T-2464
  • CAS:54827-17-7
  • Formula:C16H20N2
  • MW:240.35
  • Appearance:Liquid
  • Purity:N/A

Starting at $101.37

Product Name Qty
TMB, Ultrasensitive
100 mL
$101.37
3,3'5,5'-Tetramethylbenzidine (TMB) has been shown to be a safe-sensitive substrate for the assay of horseradish peroxidase (HRP). Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is formed. This compound, a cation free radical, is blue in color with an adsorption maximum at 653 nm. Further reaction with HRP/H, or acidification of the radical with acid yields the diimine terminal oxidation product adsorbing light at 450 nm. The extinction coefficient of the radical (E653 nm = 3.9 x 10 mo⁻1 cm⁻) and diimine (E450 nm = 5.9 x 10 mol⁻ cm⁻) provide a remarkably sensitive system for the assay of HRP and HRP labeled probes. TMBUS, available from Biovision Inc., is a single component reagent stable at room temperature and not sensitive to normal laboratory light. It is optimized with respect to TMB and hydrogen peroxide concentrations and yields a linear response with the concentrations of HRP usually employed in immunologic assays.
More Information
Alternate Name/Synonyms
3,3',5,5'-Tetramethylbenzidine; TMBUS
SKU
T-2464
CAS #
54827-17-7
Chemical Name
3,3',5,5'-Tetramethylbenzidine; TMBUS
Chemical Formula
C16H20N2
Molecular Weight
240.35
Appearance
Liquid
Storage Temp
+4°C
THERAPEUTIC AREA
Not Available
USE
TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays
MDL NUMBER
MFCD00007748
CHEMACX
X1009100-2
INCHI
InChI=1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
SMILES
Cc1cc(cc(c1N)C)c2cc(c(c(c2)C)N)C
Peptide sequence
Not Applicable
RTECS
DV2300000
UN #'S
Not Applicable
PACKING GROUP
Not Applicable
HAZARD CLASS
Not Applicable
Handling
Protect from light
Handling Protect from light
UN #'S Not Applicable
Packing Group Not Applicable
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