Trichostatin A, TSA

SKU
T-1052
  • CAS:58880-19-6
  • Formula:C17H22N2O3
  • MW:302.4 Da
  • Appearance:Beige powder
  • Purity:≥98%

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Product Name Qty
Trichostatin A
1 mg
$104.65
Trichostatin A
5 mg
$418.61

Description

Trichostatin A (TSA), is an antifungal antibiotic isolated from Streptomycs platensis. It’s cytostatic and differentiating properties are utilized in mammalian cell culture. It is a potent and specific, nonselective HDAC inhibitor, shown to have anti-inflammatory and neuroprotective properties. It inhibits all class I and class II HDAC’s and induces histone acetylation. TSA interacts reversibly with the HDAC catalytic site preventing binding of the substrate. HDACs - Classes I, II, and IV all require a zinc molecule as an essential cofactor in their active site and are inhibited by Zn2+-binding HDAC inhibitor - Trichostatin A (TSA). 

Studies have shown antiproliferative and HDAC inhibitory activity of TSA in vitro, in human breast cancer cell lines. its antitumor efficacy and toxicity has been studied in vivo in a carcinogen-induced rat mammary cancer model, which provided evidence for potent dose-dependent antitumor activity of TSA against breast cancer in vitro and in vivo. These studies strongly support HDAC as a molecular target for anticancer therapy in breast cancer.

 

 

 

More Information
Alternate Name/Synonyms
TSA; TSN
SKU
T-1052
CAS #
58880-19-6
Chemical Name
N/A
Chemical Formula
C17H22N2O3
Molecular Weight
302.4 Da
Appearance
Beige powder
Purity
≥98%
Solubility
May be dissolved in DMSO (15 mg/mL); or Ethanol (3 mg/mL).
Melting Point
150-151°C
Preparation
N/A
Storage Temp
-20°C, desiccated
THERAPEUTIC AREA
Inflammatory diseases
USE
Trichostatin A (TSA) is a potent and non-competitive reversible inhibitor of histone deacetylases (HDAC). In HeLa cells, TSA blocked cell cycle progression at G1 and induced a 12-fold increase in intracellular levels of gelsolin. In NIH 3T3 cells, TSA induced reversion of oncogenic ras-transformed cells to a normal morphology. In Jurkat cells, TSA inhibited IL-2 gene expression and displayed immunosuppressive activity in a mouse model. Induces increased acetylation of GATA4, a cardiac-specific transcription factor and increases cardiac muscle cell differentiation. In normal fibroblasts, induced Friend cell differentiation and inhibited the G1 and G2 phases of the cell cycle. TSA is a useful tool for induction of hyperacetylation of cellular histones and for further elucidation of their role in gene expression.
Merck Index
N/A
RTECS
MI5215000
Handling
Avoid contact with skin, eyes or clothing. Wash contaminated clothing before reuse. Do not eat, drink or smoke when using this product. Use personal protective equipment as required. Do not breathe dust/fume/gas/mist/vapors/spray. Use with local exhaust ventilation.
Citations
DEVELOPMENT/PLASTICITY/REPAIR: Epigenetic Enhancement of BDNF Signaling Rescues Synaptic Plasticity in Aging Yan Zeng, Miao Tan, Jun Kohyama, Marissa Sneddon, Joseph B. Watson, Yi E. Sun, and Cui-Wei Xie J. Neurosci., Dec 2011; 31: 17800 - 17810.
Citations_Hyperlink
http://www.jneurosci.org/cgi/reprint/31/49/17800
Certificate of Analysis 1
Certificate of Analysis 2
Certificate of Analysis 3
GHS PICTOGRAMS
Handling Avoid contact with skin, eyes or clothing. Wash contaminated clothing before reuse. Do not eat, drink or smoke when using this product. Use personal protective equipment as required. Do not breathe dust/fume/gas/mist/vapors/spray. Use with local exhaust ventilation.
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