TurboNuclease

SKU
T-1222
  • Appearance:Liquid
  • Purity:>99%

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Starting at $245.68

Product Name Qty
TurboNuclease
50,000 Units
$245.68

Description

TurboNuclease (also known as Benzonase nuclease) is a recombinant form of Serratia macescens extracellular endonuclease produced in E. coli using a proprietary process. This nonspecific endo-nuclease hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5-phosphorylated oligo-nucleotides of 1-4 bases in length. TurboNuclease is a highly purified homodimer of 27 kDa subunits that has exceptional high specific activity and is free of protease activity. TurboNuclease is ideal to digest nucleic acids and to reduce viscosity during protein purification and sample preparation.

Formulation: 250 units/µl in 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM MgCl2 and 50% Glycerol

Applications

TurboNuclease can be used to:

• reduce viscosity of cell lysate and reduce back pressure of column loading
• remove nucleic acid contamination from sample preparations, reduce nucleic acid contamination of Ni column purification
• reduce smearing in SDS-PAGE when used with 10%SDS or Gel Loading Dye to make whole cell lysate
• reduce or prevent clumping of concentrated cells and thawed cells
• replace crude DNase I in many applications

More Information
Alternate Name/Synonyms
Benzonase nuclease
SKU
T-1222
CAS #
N/A
Chemical Name
N/A
Chemical Formula
N/A
Molecular Weight
N/A
Appearance
Liquid
Purity
>99%
Solubility
N/A
Melting Point
N/A
Preparation
N/A
Storage Temp
-20°C
THERAPEUTIC AREA
N/A
USE
TurboNuclease is ideal to digest nucleic acids and to reduce viscosity during protein purification and sample preparation.
Merck Index
N/A
MDL NUMBER
MFCD00131010
CHEMACX
X1837864-8
INCHI
N/A
SMILES
N/A
Peptide sequence
N/A
RTECS
N/A
UN #'S
N/A
PACKING GROUP
N/A
HAZARD CLASS
N/A
Handling
TurboNuclease is stable in the storage buffer at 37°C for at least one week without any loss of activity. It can be flash-frozen in liquid nitrogen for long term storage at -70°C.
Certificate of Analysis 1
Certificate of Analysis 2
Certificate of Analysis 3
Handling TurboNuclease is stable in the storage buffer at 37°C for at least one week without any loss of activity. It can be flash-frozen in liquid nitrogen for long term storage at -70°C.
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