Beta-Lactamase, Extended Spectrum

SKU
L-2467
  • CAS:9073-60-3
  • Appearance:Freeze dried powder

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Product Name Qty
Beta-Lactamase, Extended Spectrum
1 KU
$235.47

DESCRIPTION

Beta-lactamases are enzymes produced by bacteria (Also known as Penicillinase) that provide resistance to β-lactam antibiotics such as penicillins, cephamycins, and carbapenems (ertapenem), although carbapenems are relatively resistant to beta-lactamase. Beta-lactamase provides antibiotic resistance by breaking the antibiotics structure.

These antibiotics all have a common element in their molecular structure: a four-atom ring known as a β-lactam. Through hydrolysis, the lactamase enzyme breaks the β-lactam ring open, deactivating the molecule's antibacterial properties.Beta-lactam antibiotics are typically used to treat a broad spectrum of Gram-positive and Gram-negative bacteria. Beta-lactamases produced by Gram-negative organisms are usually secreted, especially when antibiotics are present in the environment.

β-LACTAMASE-BS is a freeze-dried product containing buffer salts and zinc. β-LACTAMASE-BS has a broad spectrum activity of complete inactivation over a broad range of cephalosporins and penicillins. 

 

APPLICATIONS

• Testing sterility of blood cultures - Blood cultures are routinely prepared in order to test for bacterial infection. False negative results might be obtained where the blood sample contains antibiotics. Incorporation of β-Lactamase in the culture medium will overcome this problem when cephalosporins/penicillins are present.

• Testing for contamination of drugs by antibiotics - US Code of Federal regulations states that “If a reasonable possibility exists that a non-penicillin drug product has been exposed to cross-contamination with penicillin, the non-penicillin drug product shall be tested for the presence of penicillin” (21 CFR 211.176, Penicillin Contamination, FDA, BY-Lines No. 8 November 1977).

• Environmental monitoring of antibiotic manufacturing area.

• Sterility testing of Bulk antibiotics as described by US Pharmcopeia (USP) Chapter 71 and EP Section 2.6.

 

More Information
Alternate Name/Synonyms
Beta-Lactamase ES; β-Lactamase II; Cephalosporinase; Penicillin amido-β-lactam hydrolase
SKU
L-2467
CAS #
9073-60-3
Chemical Name
(6R,7R)-3-[(3-carboxy-4-nitrophenyl)sulfanylmethyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
Chemical Formula
N/A
Molecular Weight
N/A
Appearance
Freeze dried powder
Purity
N/A
Solubility
Soluble in water.
Melting Point
N/A
Preparation
N/A
Storage Temp
4°C
THERAPEUTIC AREA
Not Available
USE
Beta-Lactamase can be used to reduce levels of penicillin in patients with severe penicillin reactions. It is used in the production of penicillin. Penicillinase from Bacillus cereus has been used in a comparison study with penicillinase from Bacillus subtillis where the Michaelis constant, shape of the Ph/activity curve and immunological properties were shown to be significantly different.
Merck Index
N/A
Handling
Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
Certificate of Analysis 1
Certificate of Analysis 2
Certificate of Analysis 3
GHS PICTOGRAMS
Handling Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
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