SDS
CofA
Description
Brain Heart Infusion Broth (BHI broth) is a useful growth medium for many types of microorganisms, including Streptococci, Pneumococci, and Meningococci just to name a few. BHI growth medium is commonly used in food safety, water safety, and antibiotic sensitivity tests. It is particularly useful for cultivating rare microbe species. Growth of anaerobic organisms can be enhanced by adding 0.1% agar to the BHI broth.
Microbe Compatibility
| Species | American Type Culture Collection # | Compatibility Range |
| Aspergillus niger | ATCC 16404 | Poor - Very Good |
| Candida albicans | ATCC 10231 | Good - Excellent |
| Enterococcus faecalis | ATCC 29212 | Good - Excellent |
| Escherichia coli | ATCC 25922 | Good - Excellent |
| Neisseria meningitides | ATCC 13090 | Fair - Good |
| Pseudomonas Aeruginosa | ATCC 27853 | Good - Excellent |
| Saccharomyces cerevisiae | ATCC 9763 | Good - Excellent |
| Staphylcoccus Aureus | ATCC 25923 | Very Good - Excellent |
| Streptococcus pneumoniae | ATCC 6305 | Good - Excellent |
| Streptococcus pyogenes | ATCC 19615 | Good - Excellent |
BHI Broth Composition
• Brain Heart Infusion from solids: 17.5 g/L
• Pancreatic Digest of Gelatin: 10.0 g/L
• Dextrose: 2.0 g/L
• Sodium Chloride: 5.0 g/L
• Disodium Phosphate: 2.5 g/L
Specifications
• pH (3.7% H2O @ 25°C): 7.2 - 7.6
• Raw Material Species: Bovine
• Enzyme Species: Porcine
More Information
Alternate Name/Synonyms
Brain heart infusion broth
SKU
B-2587
Appearance
Homogeneous free-flowing, beige powder
Solubility
Water
Storage Temp
Room temperature, protect from moisture and light
THERAPEUTIC AREA
Not Applicable
USE
Growth medium for fastidious pathogenic microorganisms; including streptococci, pneumococci, and meningococci. Often used in food and water safety and antibiotic sensitivity tests.
Handling
Use in a chemical fume hood, with air supplied by an independent system. Avoid inhalation, contact with eyes, skin and clothing. Avoid the formation of dust and aerosols. Use in a well-ventilated area. Keep away from sources of ignition. Avoid prolonged or repeated exposure.
Certificate of Analysis 1
| Handling | Use in a chemical fume hood, with air supplied by an independent system. Avoid inhalation, contact with eyes, skin and clothing. Avoid the formation of dust and aerosols. Use in a well-ventilated area. Keep away from sources of ignition. Avoid prolonged or repeated exposure. |
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